Isolation and screening of endophytic bacteria from strawberry runner with plant growth promotion properties and antifungal activity against some fungi
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Abstract
The objective of this research was to isolate endophytic bacteria from strawberry runners and to screen for plant growth-promoting properties and antifungal activity against some fungi. A total of 117 isolates of bacterial endophytes showed soluble phosphate activity when tested by the Molybdenum-blue method. Isolate DB021 had the highest phosphate solubility efficiency at 234.64±2.64 µg/ml. Also, isolate DB041 had the highest IAA production of 322.10±0.87 µg/ml when tested with the Salkowski's Reagent. Dual culture assay indicated that antagonism of DB065 against Rhizoctonia solani 1406 had the highest percentage of inhibition (42.67±1.59). Furthermore, DB071 inhibited growth of Fusarium sp. FB3-3 and Colletotrichum gloeosporioides DBCS-01 by 43.05±0.18% and 54.92±0.26%, respectively. In this study, the cell-free supernatant obtained from five different media were tested against Rhizoctonia solani 1406, Fusarium sp. FB3-3 and C. gloeosporioides DBCS-01. The agar well diffusion assay showed that DB071 cultivated in T2 medium inhibited mycelial growth of the three fungal pathogens. Based on 16S rRNA gene sequence analysis, eight bacterial isolates were classified as follows: Bacillus subtilis DB016, B. siamensis DB051 and DB071, Enterobacter quasiroggenkampii DB035, Enterobacter sp. DB041 and DB044, Microbacterium enclense DB021, and Pseudomonas aeruginosa DB065. This is the first in vitro study of endophytic bacteria isolated from strawberry runners with the potential to enhance plant growth. The most promising isolates will now be evaluated for their effectiveness to reduce disease and promote strawberry production based on Good Agricultural Practices (GAP).
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คลินิกพืช กลุ่มพัฒนาการตรวจสอบพืช และปัจจัยการผลิต สำนักวิจัย และพัฒนาการเกษตร เขตที่ 1. 2562. โรคเหี่ยวสตรอเบอรี่. จาก แหล่งข้อมูล: http://oard1.doa.go.th/pdf. สืบค้นเมื่อวันที่ 2 กุมภาพันธ์ 2565.
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