Properties and stability of probiotic Bacillus spores in chicken feed
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Abstract
The objective of this research was to study the properties and persistence of probiotic Bacillus spore under different conditions of 4 bacteria isolates of namely B. aryabhattai (CKNJh11), THPS1, PWR01, and OYNH19 which were separated from shrimp pond soil, hot spring soil, cup lump rubber and chicken feces. The 4 isolates had 97.05, 92.21, 92.08, and 84.58% of sporulation efficiency, respectively. In the gastric acid tolerance test the survival rate was greater than 83%. Bile salt tolerance had a higher survival rate than 73% and the isolate OYNH19 spores were the best heat resistant at 80 °C, with a survival rate of 91.30%. B. aryabhattai (CKNJh11), THPS1 and PWR01 had the best heat resistance at 70 °C, with survival rates of 85.94, 90.10 and 90.79%, respectively. The ability of tolerances to alcohol lysozyme and salinity of 4 isolates had higher survival rates of more than 70%. When the four isolates of the bacterial spores were mixed in chicken feed and kept at various temperatures for 30 days, the bacterial spores of the 4 isolates showed a survival rate of more than 70%. The results of this study showed that the Bacillus spores tested in all 4 isolates were stable, durable, and resistant to various conditions. It can be applied to use as probiotics in agriculture, animal husbandry, and the feed industry.
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References
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