Gas chromatography mass spectrometry (GC-MS) data detect on metabolites in chilli seedlings induced by Bacillus subtilis B01
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Abstract
Secondary metabolites data of genus Bacillus induced plant can support the evidence of plant response to the biological control agent for plant disease management. In this study, Bacillus subtilis isolates B01 were identified by VITEK-2 system with BCL card and a good identity level at 91% probability. The causal of anthracnose fungi, Colletotrichum truncatum and C. gloeosporioides were tested by dual culture method. The result showed fungal inhibition at 69.71% and 60%. B. subtilis gave the higher seed germination rate with 90%, while 84% in nontreated chilli seeds. During the stage of true leaves at 16 days-old chilli seedlings, height and root length were longer than the control. Consequently, the result leads to the seed vigor index of bacterial treated was higher than the control (690.58 and 514.42 respectively). The induction result of secondary metabolite chilli after seed soaking with B. subtilis isolate B01 at 16 days-old was analyzed by Gas chromatography-mass spectrometry (GC-MS). The compound fragment and mass were compared with data from the National Institute of Standards and Technology database. The compound information was referred from PubChem database. The induced secondary metabolites from chilli seedlings were compared to the control. The result showed that the cell and microorganism growth inhibited substances were 1H-Imidazole, 4-methyl-; 6-Ethoxy-1,2,3,4-tetrahydro-2,2,4- trimethylquinoline; Cyclodecanamine; Heneicosane and Eicosane, 1-iodo-. Furthermore, the secondary metabolites related to growth-promoting and volatile oil-forming groups were Hexadecane; Piperidine, 2-propyl-; Undecane 3,8-dimethyl-; 2-Bromo dodecane and Nonane, 2,2,4,4,6,8,8-heptamethyl-. Lastly, we can observe an essentially industrial bioactive compound as Iron pentacarbonyl.
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