Screening and efficacy of endophytic bacteria for control root and stem rot of durian caused by Phytophthora palmivora in greenhouse conditions
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Abstract
The root and stem disease of durian caused by Phytophthora palmivora is a serious agent that infects root and stem, leaves and fruit reducing yield loss. This research aimed to select the endophytic bacteria for their control root and stem rot of durian in laboratory and greenhouse conditions. The samples of root and stem disease were collected from root and soil in durian plantations. Whereas, endophytic bacteria were collected from leaves of healthy durian at Changklang District, Nakhon Si Thammarat Province. The results showed 3 isolates of Phytophthora and 14 isolates of endophytic bacteria were found. While, 4 isolates of endophytic bacteria, BS003, BS013, BS011 and BS006 show high efficacy in inhibition P. palmivora obtained 68.60, 66.50, 65.20 and 62.90%, respectively. The efficacy of 50 ml/tree cell suspensions of antagonistic endophytic bacteria for controlling root and stem rot of durian in greenhouse conditions showed highly effective. The use of B. subtilis BS013, disease incidence (%DI) was the least obtained 6.20%. Use of Bacillus BS006, the disease severity index (%DSI) showed the lowest obtained 0.75% and use of Bacillus BS011, the total populations of bacteria after 30 days inoculated were high obtained 1.21 x 107 cfu/g soil.
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