Effect of elicitor substances on bioactive compound content of Phaya Yo (Clinacanthus nutans (Burm.f.) Lindau) culture in vitro
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Abstract
Phaya Yo (Clinacanthus nutans (Burm.f.) Lindau.) is a fascinating and significant medicinal plant raw ingredients that are of high quality, uncontaminated, consistent in quantity, and predictable are required for the manufacturing of herbal medicines. The objective of this study is to modify in vitro plant culture techniques and stimulate bioactive compounds of Phaya Yo “Dong Bang” (Clinacanthus nutans (Burm.f.) Lindau). Nodal explants of Phaya Yo were cultured on MS medium supplemented with 0.5 mg/L TDZ and 0.1 mg/L NAA in combination with yeast extract (YE) at concentrations of 0.5, 1.0, 1.5, and 2 g/L and compared with methyl jasmonate (MeJA) at concentrations of 10, 20, 30, and 40 µM. It was found that nodal explants were cultured on MS medium supplemented with 0.5 mg/L TDZ and 0.1 mg/L NAA in combination with 40 µM methyl jasmonate gave the greatest total phenolic content and radical scavenging activity DPPH with an amount of 22.69 mg GAE/g DW (4.08-fold of plant leaf sample from in the pot) and 32.29 mg TroloxE/g DW (6.90-fold of plant leaf sample from in the pot), respectively. For phytosterols, it was found that both types of stimulants do not affect increasing the amount of phytosterols. The highest amount of lupeol was 1.46 mg/g DW in the medium supplemented with methyl jasmonate (MeJA) at concentrations of 10 µM.
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References
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