Supplementation of antioxidants to enhance quality of frozen semen and alleviate lipid peroxidation of Boer goat
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Abstract
This research aimed to investigate the effects of different concentrations of antioxidants glutathione (GSH), cysteine, and vitamin E) on the cryopreservation of Boer goat sperm. The sperm was diluted with a tris-based extender and different concentrations of each antioxidant with respective concentrations (1, 3 and 5 mM for GSH), (6, 9 and 12 mM for cysteine) and (1, 2 and 3 mM for vitamin E) were supplemented. Extender with no antioxidant supplementation was also tested, and Andromed was used as control. A completely randomized design (CRD) with six replications was examined for each semen parameter. Straws were frozen in liquid nitrogen vapor. After storage for ten days, the frozen straws were thawed and evaluated for sperm quality. The results showed that the addition of 1 mM GSH in tris-based semen extenders yielded higher membrane integrity and acrosome integrity compared to the other treatments (P<0.05). Furthermore, adding 1 mM GSH or 1 mM vitamin E to the buck semen extender decreased the lipid peroxidation of the frozen semen (3.01 and 2.96 nmol/mL, respectively). These showed significantly better results than the other treatment groups at P<0.05 but did not show a significant difference to Andromed (P>0.05). Nevertheless, supplementation with 1 mM GSH did not show a significant difference in DNA methylation compared to that of Andromed (P>0.05) and yielded a fertilization rate of 66.67% (n=6/9) compared to Andromed 37.50% (n=3/8). Thus, this study suggests that 1 mM of GSH can be considered a suitable alternative antioxidant for semen cryopreservation of Boer goat, which can increase goat production in the future.
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