Polymorphism and association study of candidate genes on Salmonella fecal shedding in pigs
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Abstract
The objective of this study was to investigate genetic polymorphisms in four candidate genes, consisting of: Chaperonin Containing TCP1 Subunit 7 (CCT7), Alpha-1-Fucosyltransferase (FUT1), Neutrophil Cytosolic Factor 2 (NCF2), and Toll-like Receptor 4 (TLR4), and their association with fecal scores and Salmonella fecal shedding in pigs. In this study, 40 crossbred pigs (50% Duroc × 25% Large White × 25% Landrace) aged 15-21 days were orally inoculated with Salmonella Typhimurium and monitored for fecal scores and Salmonella fecal shedding at 2, 7, 14, and 21 days post-inoculation (dpi). Polymorphisms in the four candidate genes were genotyped using the PCR-RFLP method. Association analysis between functional candidate genes and diarrhea scores was performed using the Cochran-Mantel-Haenszel test, while Salmonella shedding levels were analyzed using the General Linear Model (GLM) procedure. The results showed no significant differences in fecal scores among pigs at any time point post-inoculation (P > 0.05). The polymorphism of CCT7 gene showed three genotypic variations, and FUT1 gene showed two genotypic variations. Meanwhile, the NCF2 gene and TLR4 gene were monomorphic. Association analysis showed that there is no significant between fecal score and genotype pattern of CCT7 and FUT1 but the polymorphisms of CCT7 gene significant associations with salmonella fecal shedding. Genotype AA had highest salmonella fecal shedding at 2 dpi (3.05 x104 CFU/ml). Therefore, the CCT7 gene may serve as a candidate marker for genetic improvement of Salmonella infection resistance in pigs.
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