Effect of mycotoxin binder on growth performance, immune function and oxidative stress of broilers chickens fed aflatoxin B1 contaminated diets under high stock density
Article Sidebar
Main Article Content
Abstract
This study investigated the effects of mycotoxin binders on growth performance, immune function and oxidative stress of broiler chickens fed aflatoxin B1 contaminated diets under high stock density. A total of 576 one-day-old male Ross 308 broiler chicks were randomly allocated to 4 treatments (8 replicates per treatment) under high stock density (18 birds/m2) in a 37-day experiment. The experimental diets consisted of a basal diet (control; CT), a basal diet contaminated with 80 µg/kg of AFB1 (Aflatoxin; AF), AF supplemented with mycotoxin binder 1 (MTB1), and AF supplemented with mycotoxin binder 2 (MTB2). Both mycotoxin binders were added at 1.0 kg/ton in the starter diet and 0.5 kg/ton in the grower and finisher diets, respectively. Feed and water were provided ad-libitum throughout the experiment. During the starter phase, the birds fed AF diet had a higher feed intake (FI) and feed conversion ratio (FCR) when compared to control groups (P<0.05). No significant difference was observed in growth performance of broilers among dietary treatments in the other phase (P>0.05). There was no significant difference in MDA of broiler chicken among dietary treatments (P>0.05). Birds fed CT, MTB1 and MTB2 had significantly higher NDV HI antibody titer when compared to that of the AF group (P<0.05). It could be concluded that the inclusion of both mycotoxin binders enhanced immune function in broilers consuming an aflatoxin B1 contaminated diet.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
กรมปศุสัตว์. 2559. เกณฑ์มาตรฐานพระราชบัญญัติควบคุมคุณภาพอาหารสัตว์ ปีงบประมาณ 2560.
วราพร ไกรถวิล, เสกสม อาตมางกูรและยุวเรศ เรืองพานิช. 2558. ผลของการเสริมสารประกอบกลูโคแมนแนนต่อการเจริญเติบโต และน้ำหนักอวัยวะภายในของไก่เนื้อที่ได้รับอาหารที่มีการปนเปื้อนสารพิษจากเชื้อรา. เรื่องเต็มการประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 53: สาขาพืช, สาขาสัตว์, สาขาสัตวแพทยศาสตร์, สาขาประมง, สาขาส่งเสริมการเกษตรและคหกรรมศาสตร์. น.852-858. ใน: การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 53. สำนักงานกองทุนสนับสนุนการวิจัย.
Adeogun, J., O. Abu, and E. Ewuola. 2022. Diet supplementation with yeast beta-glucans, dietary antioxidants and vitamin k in broiler chickens mitigated aflatoxin-induced growth retardation. Slovak Journal of Animal Science. 55: 14-26.
Alayande, L., G. Bawa, N. Rano, and S. Ogundipe. 2023. Response of broiler chicken fed aflatoxin maize-based diets supplemented with various levels of mycotoxin detoxifier. Slovak Journal of Animal Science. 56: 17-29.
Aviagen. 2022. Ross broiler: Nutrition specifications. The local Aviagen, US.
Balogh, K., B. Kövesi, E. Zándoki, S. Kulcsár, Z. Ancsin, M. Erdélyi, C. Dobolyi, I. Bata, K. Inotai, A. Szekeres, M. Mézes, and J. Kukolya. 2019. Effect of sterigmatocystin or aflatoxin contaminated feed on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in broiler chicken. Antioxidants (Basel, Switzerland). 8: 201.
Chen, J., K. Chen, S. Yuan, X. Peng, J. Fang, F. Wang, H. Cui, Z. Chen, J. Yuan, and Y. Geng. 2016. Effects of aflatoxin B1 on oxidative stress markers and apoptosis of spleens in broilers. Toxicology and Industrial Health. 32: 278–284.
Eraslan, G., M. Akdogan, E. Yarsan, F. Şahindokuyucu, D. Essiz, and L. Altintaş. 2005. The effects of aflatoxins on oxidative stress in broiler chickens. Turkish Journal of Veterinary and Animal Sciences. 29: 701-707.
Farooqui, M. Y., M. Rashid, S. Mehmood, and M. Malik. 2019. Aluminosilicates and yeast-based mycotoxin binders: Their ameliorated effects on growth, immunity and serum chemistry in broilers fed aflatoxin and ochratoxin. South African Journal of Animal Science. 49: 619.
Fawaz, M. A., H. A. Hassan, and A. A. Abdel. 2022. Aflatoxins in poultry feed: Present status and future concerns. SVU-International Journal of Agricultural Sciences. 4: 113-124.
Frechaut, E., E. Muchanga, P. Taunde, O. Nhambirre, A. Pondja, and C. Bila. 2015. Evaluation of serum antibody titers against Newcastle disease in broiler poultry in Maputo and Matola Regions, Mozambique. International Journal of Poultry Science. 14: 622-624.
Gholami, A. K., M. Rahimi, H. Raei, R. Majedifar, and T. M. Karimi. 2025. Research note: Protective effects of compound mycotoxin detoxifier in broiler chicken fed aflatoxins-contaminated diets. Poultry Science. 104: 104802.
Grotto, D., L. D. Santa, S. Boeira, J. Valentini, M. F. Charão, A. M. Moro, P. C. Nascimento, V. J. Pomblum, and S. C. Garcia. 2007. Rapid quantification of malondialdehyde in plasma by high performance liquid chromatography-visible detection. Journal of Pharmaceutical and Biomedical Analysis. 43: 619-624.
Hussain, Z., H. U. Rehman, S. Manzoor, S. Tahir, and M. Mukhtar. 2016. Determination of liver and muscle aflatoxin B1 residues and select serum chemistry variables during chronic aflatoxicosis in broiler chickens. Veterinary Clinical Pathology. 45: 330–334.
Insawake, K., T. Songserm, O. Songserm, Y. Theapparat, K. D. Adeyemi, K. Rassmidatta, and Y. Ruangpanit. 2025. Influence of phytochemicals on growth performance, gut morphology and ceca microbiome in broilers fed aflatoxin-contaminated diet and raised under high stocking density and heat stress. Poultry Science. 104: 105293.
Kamboh, A. A., S. Q. Hang, M. A. Khan, and W. Y. Zhu. 2016. In vivo immunomodulatory effects of plant flavonoids in lipopolysaccharide-challenged broilers. An International Journal of Animal Bioscience. 10: 1619–1625.
Kolawole, F., S. Oyeyinka, B. Mutiat, and F. Oluwabiyi. 2020. Chemical composition and consumer acceptability of agidi (maize gel) enriched with orange-fleshed sweet potato and soybean. Ceylon Journal of Science. 49: 463.
Lai, Y., M. Sun, Y. He, J. Lei, Y. Han, Y. Wu, D. Bai, Y. Guo, and B. Zhang. 2022. Mycotoxins binder supplementation alleviates aflatoxin B1 toxic effects on the immune response and intestinal barrier function in broilers. Poultry Science. 101: 101683.
Li, M. H., E. H. Robinson, D. F. Oberle, P. M. Lucas, and B. G. Bosworth. 2012. Evaluation of corn gluten feed and cottonseed meal as partial replacements for soybean meal and corn in diets for pond-raised hybrid catfish, Ictalurus punctatus×I. furcatus. Journal of the World Aquaculture Society. 43: 107-113.
Liu, X., C. Xu, Z. Yang, W. Yang, L. Huang, S. Wang, F. Liu, M. Liu, Y. Wang, and S. Jiang. 2020. Effects of dietary zearalenone exposure on the growth performance, small intestine disaccharidase, and antioxidant activities of weaned gilts. Animals: an open access journal from MDPI. 10: 2157.
Manegar, G. A., B. E. Shambulingappa, and K. J. Ananda. 2010. Studies on tolerance limit of aflatoxin in commercial broilers. Libyan Agriculture Research Center Journal International. 1: 177-181.
Nalle, C., A. Angi, M. Supit, S. Ambarwati, and N. Yuliani. 2019. Respone of broiler chickens fed on diets containing low level of aflatoxin (B1 and B2) and supplemented with mtcotoxin binder. Jurnal Kajian Veteriner. 7: 98-102.
Ochieng, P. E., S. Croubels, D. Kemboi, S. Okoth, D. S. Baere, E. Cavalier, E. Kang'ethe, J. Faas, B. Doupovec, J. Gathumbi, C. Douny, M. L. Scippo, J. F. Lindahl, and G. Antonissen. 2023. Effects of aflatoxins and fumonisins, alone or in combination, on performance, health, and safety of food products of broiler chickens, and mitigation efficacy of bentonite and fumonisin esterase. Journal of Agricultural and Food Chemistry. 71: 13462–13473.
Oğuz, H., V. Kurtoğlu, and B. Coşkun. 2000. Preventive efficacy of clinoptilolite in broilers during chronic aflatoxin (50 and 100 ppb) exposure. Research in Veterinary Science. 69: 197–201.
Pappas, A. C., E. Tsiplakou, D. I. Tsitsigiannis, M. Georgiadou, M. K. Iliadi, K. Sotirakoglou, and G. Zervas. 2016. The role of bentonite detoxifiers in single or concomitant mycotoxin contamination of chicken diets. British Poultry Science. 57: 551-558.
Paraskeuas, V., E. Griela, D. Bouziotis, K. Fegeros, G. Antonissen, and K. C. Mountzouris. 2021. Effects of deoxynivalenol and fumonisins on broiler gut cytoprotective capacity. Toxins. 13: 729.
Rodsatian, N., O. Songserm, I. Peñarrubia, M. Serra, J. Crespo, A. Blanch, and Y. Ruangpanit. 2023. Effect of dietary supplementation of citrus flavonoids on performance, intestinal epithelium morphology, microbiota in excreta and oxidative stress of broiler chickens subjected to heat stress. European Poultry Science. 87: 1-13.
Sarker, M. T., X. L. Wan, H. M. Yang, and Z. Y. Wang. 2023. AflatoxinB1 (AFB1) and its toxic effect on the broilers intestine: A review. Veterinary Medicine and Science. 9: 1646–1655.
SAS Institute Inc. 2021. SAS®OnDemand for academics: Studio. SAS Institute Inc. Cary, North Carolina, USA.
Seomoon, K. M., and I. S. Jang. 2022. Application of plant flavonoids as natural antioxidants in poultry production. Korean Journal of Poultry Science. 49: 211-220.
Shen, N., T. Wang, Q. Gan, S. Liu, L. Wang, and B. Jin. 2020. Plant flavonoids: classification, distribution, biosynthesis, and antioxidant activity. Food Chemistry. 383: 132531.
Tao, W., W. Zhu, F. Nabi, Z. Li, and J. Liu. 2023. Penthorum chinense Pursh compound flavonoids supplementation alleviates aflatoxin B1-induced liver injury via modulation of intestinal barrier and gut microbiota in broiler. Ecotoxicology and Environmental Safety. 255: 114805.
Terra, X., A. Ardevol, M. Blay, M. Pinent, and J. Salvadó. 2017. Chapter 26 - Flavonoids as Protective Agents Against Diet-Induced Oxidative Damage at Gastrointestinal Tract. p.327-338. In J. Gracia-Sancho and J. Salvadó (Eds.). Gastrointestinal Tissue. Academic Press, CA.
Williamson, G. 2020. Dietary flavonoids. Present Knowledge in Nutrition. 561-572.
Yagi, K. 1976. A simple fluorometric assay for lipoperoxide in blood plasma. Biochemical Medicine. 15: 212-216.
Zabiulla, I., V. Malathi, H. Swamy, J. Naik, L. Pineda, and Y. Han. 2021. The efficacy of a smectite-based mycotoxin binder in reducing aflatoxin B1 toxicity on performance, health and histopathology of broiler chickens. Toxins. 13: 856.
Zhang, J., Y. Fang, Y. Fu, S. Jalukar, J. Ma, Y. Liu, Y. Guo, Q. Ma, C. Ji, and L. Zhao. 2023. Yeast polysaccharide mitigated oxidative injury in broilers induced by mixed mycotoxins via regulating intestinal mucosal oxidative stress and hepatic metabolic enzymes. Poultry Science. 102: 102862.
Zhao, Y., P. Zhang, H. Fang, C. Guo, B. Zhang, and F. Wang. 2021. Bentonite-assisted microbial-induced carbonate precipitation for coarse soil improvement. Bulletin of Engineering Geology and the Environment. 80.
Zou, Y., S. B. Liu, Q. Zhang, and H. Z. Tan. 2023. Effects of Aflatoxin B1 on growth performance, carcass traits, organ index, blood biochemistry and oxidative status in Chinese yellow chickens. The Journal of Veterinary Medical Science. 85: 1015-1022.
Zou, Z., W. Xi, Y. Hu, C. Nie, and Z. Zhou. 2016. Antioxidant activity of citrus fruits. Food Chemistry. 196: 885–896.
