Screening of resistance genes using DNA markers and evaluation of resistance level to brown plant hoppers in local rice varieties grown in some areas of lower northern Thailand
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Abstract
Brown planthopper (Nilaparvata lugens) is one of the most destructive insect pests of rice in Thailand. This insect has been adapting to their environment, making it difficult to control. Thailand is considered one of the rice genetic resources, especially local rice varieties, which are the source of important insect resistance genes and can be used in rice breeding programs for insect resistant varieties. This study aimed to determine brown planthopper resistance genes using gene-specific primers (Bph3, Bph14, and Bph18(t)) and evaluate resistance levels in 20 local rice varieties. The results showed that all 20 local rice varieties have at least one brown planthopper resistance gene, and two rice varieties i.e., Hom Dong and Lon Khrok rice carried all three brown planthopper resistance genes (Bph3, Bph14, and Bph18(t)). The evaluation for resistance to brown planthopper infestation showed that Hom Dong rice had the resistance levels (R) and moderately resistant (MR) on the 7th and 14th days after infestation, respectively. But the level of resistance decreased to susceptible level in Lon Khrok rice. Meanwhile, Lueang Kaset and Khao Chalo rice had the same level of resistance as Hom Dong rice but contained only one resistance gene (Bph3). Each local rice variety statistically differs in levels of resistance. Therefore, local rice varieties tested here showed the genetic variations that were beneficial for breeding programs on rice resistance varieties against brown plant hoppers.
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References
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