Effects of Carriers on Efficiency of Antagonistic Bacteria Bacillus subtilis and Bacillus amyloliquefaciens on Inhibition of Rigidoporus microporus causing of White Root Disease in Para Rubber
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Abstract
Rigidoporus microporus is a soil-borne fungus that causes white root rot disease of para rubber. Controlling disease using bio-agent is effective and safe for environment. In addition to antagonistic bacteria, carrier is a key component of bio-agent. Therefore, this research aimed to study the effect of carriers on efficacy of antagonistic bacteria Bacillus subtilis isolate SM1, LPDD3-2 and Bacillus amyloliquefaciens isolate PT7 for inhibition of R. microporus. Seven carriers including bentonite, kaolin, lactose, modified starch, rice starch, cassava starch and corn starch were tested in completely randomized design (CRD) for 4 replications. The result showed that antagonistic bacteria on potato dextrose agar (PDA) containing bentonite showed the highest viability and different from other carriers (P ≤ 0.01). Including, the antagonistic bacteria revealed enlarge size of colony in this medium. The mycelial growth of R. microporus on PDA containing bentonite was not statistically different from the control (PDA). R. microporus growth inhibition by B. subtilis isolate SM1 and LPDD3-2 on PDA containing bentonite was higher than other carriers using dual culture method and different from the control (PDA) (P ≤ 0.01). The result indicated that carriers affected the growth and efficacy of antagonistic bacteria. The selection of the proper carrier is critical to the development of antagonistic bacteria bio-agent.
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