Potential of Entomopathogenic Fungi Isolated from Soils in Uttaradit Province for Controlling Diamondback Moth Larva
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Published:
Jul 10, 2019
Keywords:
Metarhizium anisopliae diamondback moth control
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Abstract
Diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae), is a serious pest of cruciferae worldwide. Therefore, this study aims to find and screen the effectively entomopathogenic fungi to control diamondback moth from forest soil in Uttaradit province. The total of 36 soil samples from five districts was collected and brought back to the laboratory. Metarhizium anisopliae at the amount of 28 isolates was identified and coded the numbers and their sources. The efficacy of those was determined by dipping the 3rd instar larva of diamondback moth in each spore suspension of fungal isolate at the concentration of 108 conidia/ml. The experiment was based on a completely randomized design (CRD) with 3 replications. The result showed that four isolates of M. anisopliae; U-NM02, U-LM01, U-PM01 and U-NM05; were high efficacy to control diamondback moth larva with 100% mortality in 4 days after dipping and LT50 were 1.80, 2.08, 2.12 and 2.23 days, respectively.
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Research Articles
References
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Sumaetha, K. and Y. Chanbang. 2014. Fitness cost of resistance to diamide insecticide in diamondback moth. Journal of Agriculture 30(1): 29-37. (in Thai)
Thongcharoen, K., W. Pongprasert, S. Buranapanichpan and J. Tayutivutikul. 2009. Efficiency of certain insecticides on diamondback moth in lower northern Thailand. Journal of Agriculture 25(3): 245-255. (in Thai)
Chui-chai, N., P. Krutmuang, S. Nalumpang, S. Mekchay, C. Khanongnuch and Y. Chanbang. 2012. Insecticidal activity and cuticle degrading enzymes of entomopathogenic fungi against Plutella xylostella (Lepidoptera: Plutellidae). CMU Journal of Natural Sciences 11(1): 147-155.
Department of Mineral Resources. 2008. Area Classification for Mineral Resource Management. Advance Vision Service Ltd., Bangkok. 97 p. (in Thai)
Dong, T., B. Zhang, Q. Weng and Q. Hu. 2016. The production relationship of destruxins and blastopores of Metarhizium anisopliae with virulence against Plutella xylostella. Journal of Integrative Agriculture 15(6): 1313-1320.
Duarte, R.T., K.C. Goncalves, D.J.L. Espinosa, L.F. Moreira, S.A. De Bortoli, R.A. Humber and R.A. Polanczyk. 2016. Potential of entomopathogenic fungi as biological control agents of diamondback moth (Lepidoptera: Plutellidae) and compatibility with chemical insecticides. Journal of Economic Entomology 109(2): 594-601.
Fernandes, E.K.K., C.A. Keyser, D.E.N. Rangel, R.N. Foster and D.W. Roberts. 2010. CTC medium: A novel dodine-free selective medium for isolating entomopathogenic fungi, especially Metarhizium acridum, from soil. Biological Control 54: 197-205.
Furlong, M.J, D.J. Wright and L.M. Dosdall. 2013. Diamondblack moth ecology and management: problems, progress, and prospects. Annual Review of Entomology 58: 517-541.
Humber, R.A. 2005. Entomopathogenic fungal identification. (Online). Available: https:// www.ars.usda.gov/ARSUserFiles/80620520/apswkshoprev.pdf (May 29, 2018).
Ismail, M., W. Wakil, N.H. Bashir, W. ul Hassan and U.W. Muhammad. 2017. Entomocidal effect of entomopathogenic fungus Beauveria bassiana and new chemistry insecticides against Spodoptera litura (Fabricius) (Noctuidae: Lepidoptera) under controlled conditions. International Journal of Agriculture Innovations and Research 5(6): 1056-1061.
Kunasakdakul, K. 2013. Plant Pathology Techniques. Chiang Mai University Press, Chiang Mai. 212 p. (in Thai)
Lacey, L.A., D. Grzywacz, D.I. Shapiro-Ilan, R. Frutos, M. Brownbridge and M.S. Goettel. 2015. Insect pathogens as biological control agents: Back to the future. Journal of Invertebrate Pathology 132: 1-41.
Nguyen, L.T. and C.T.B. Vo. 2007. Biocontrol potential of Metarhizium anisopliae and Beauveria bassiana against diamondback moth, Plutella xylostella. Omonrice 15: 86-93.
Ozturk, H.E., O. Guven and I. Karaca. 2015. Effects of some bioinsecticides and entomopathogenic fungi on Colorado potato beetle (Leptinotarsa decemlineata L.). Communications in Agricultural and Applied Biological Sciences 80(2): 205-211.
Razkhanty, K. and S. Klangsinsirikul. 2017. Screening of potential insect pathogenic fungi for controlling cabbage aphid (Lipaphis erysimi). King Mongkut’s Agricultural Journal 35(2): 65-75. (in Thai)
Rowell, B., N. Bunsong, K. Satthaporn, S. Phithamma and C. Doungsa-ard. 2005. Hymenopteran parasitoids of diamondback moth (Lepidoptera: Ypeunomutidae) in northern Thailand. Journal of Economic Entomology 98(2): 449-456.
Shelton, A.M., J.L. Robertson, J.D. Tang, C. Perez, S.D. Eigenbrode, H.K. Preisler, W.T. Wilsey and R.J. Cooley. 1993. Resistance of diamondback moth (Lepidoptera: Plutellidae) to Bacillus thuringiensis subspecies in the field. Journal of Economic Entomology 86(3): 697-705.
Shen, J., D. Lin, S. Zhang, X. Zhu, H. Wan and J. Li. 2017. Fitness and inheritance of metaflumizone resistance in Plutella xylostella. Pesticide Biochemistry and Physiology 139: 53-59.
Silva, V.C.A., R. Barros, E.J. Marques and J.B. Torres. 2003. Susceptibility of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to the fungus Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. Neotropical Entomology 32(4): 653-658.
Sribunme, N., W. Pongprasert, S. Buranapanichpan, P. Sinchayakul and J. Kulsarin. 2018. Efficacy of some carriers to preserve Metarhizium anisopliae MRT-PCH 048 for controlling brown planthopper. Journal of Agriculture 34(2): 227-234. (in Thai)
Sripontan, Y. 2016. Comparisons on the development of cutworm, Spodoptera litura (F.) and diamondback moth, Plutella xylostella L. reared on three varieties of cruciferous plants. Songklanakarin Journal of Plant Science 3(Suppl. 3): 56-62. (in Thai)
Sumaetha, K. and Y. Chanbang. 2014. Fitness cost of resistance to diamide insecticide in diamondback moth. Journal of Agriculture 30(1): 29-37. (in Thai)
Thongcharoen, K., W. Pongprasert, S. Buranapanichpan and J. Tayutivutikul. 2009. Efficiency of certain insecticides on diamondback moth in lower northern Thailand. Journal of Agriculture 25(3): 245-255. (in Thai)
