Strain Improvement of <I>Cordyceps tenuipes</I> (Peck) for Snowflake Cordyceps Production on Silkworm Pupae
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Abstract
Snowflake cordyceps, Cordyceps tenuipes (Peck), is an entomopathogenic fungus which has been described as pharmaceutics due to its bioactive compounds in fruiting body or synnemata. Thus, the objective aimed to improve the fungal strain using ultraviolet radiation for high content of bioactive compounds such as adenosine. The result revealed that the characteristics and growth of the fungus have changed after exposing to ultraviolet C radiation of wavelength 254 nm from fluorescent lamps 30 watt (GHangqing T8, China). The colonies were white in color, thick mycelia and full grown on PDA. The colonies of QSDS1-1 isolate exposed to UVC for 0.5 and 5 minutes and QSDS1-2 isolate exposed to 0.5 and 10 minutes showed fully grown on PDA with white and thick mycelia. Then, these four selected colonies were tested for fruiting body production using a silkworm pupa as a substrate. It was obvious that the fungal isolate QSDS1-2 after exposing to UVC for 10 minutes yielded the rapid growth of yellow fruiting body with sharp at the end. There were white conidia on the terminal end of fruiting body which the average length was 2 cm. The fresh and dry weights of fruiting body were 1.10 and 0.50 g, respectively. The adenosine content from fruiting body of QSDS1-2 exposed UVC for 10 minutes was 50.11 mg/100 g.
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