วิธีการทางอณูพันธุศาสตร์สำหรับการวินิจฉัยโรคจุดขาวในกุ้งขาวแวนนาไม

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รัชชานนท์ กุศลสงเคราะห์กุล
วาสนา ศิริแสน
ปองพล พงไธสงค์
วุฒิชัย เคนไชยวงศ์

บทคัดย่อ

กุ้งขาวแวนนาไม (L. vannamei) เป็นสายพันธุ์ที่มีการเพาะเลี้ยงมากที่สุดในโลก ทำให้เมื่อเกิดโรคระบาดจะส่งผลกระทบต่ออุตสาหกรรมการเพาะเลี้ยงกุ้ง โดยมีโรคจุดขาวเป็นโรคที่สำคัญ เนื่องจากอัตราการป่วย และอัตราการตายสูง ในปัจจุบันมีวิธีการตรวจหาและวินิจฉัยโรคจุดขาวที่แนะนำ คือ ปฏิกิริยาลูกโซ่โพลิเมอเรสเพื่อวินิจฉัยเบื้องต้นและยืนยัน เนื่องจากมีความไวและจำเพาะต่อเชื้อและสามารถตรวจได้ในกุ้งเกือบทุกระยะ หากเป็นวิธีการสังเกตอาการทางคลินิก วิธีการทางมหพยาธิวิทยา จุลพยาธิวิทยา ควรได้รับการวินิจฉัยยืนยันด้วยวิธีทางภูมิคุ้มกัน หรือวิธีทางโมเลกุล เช่น การตรวจหาแอนติเจนแอนติบอดี ปฏิกิริยาลูกโซ่โพลิเมอเรส LAMP และ ISH ส่วนวิธีกล้องจุลทรรศน์อิเล็กตรอนแบบส่องผ่าน วิธีชีววิเคราะห์ และการเพาะเลี้ยงเซลล์ สามารถตรวจเพื่อยืนยันได้แต่ไม่แนะนำเนื่องจากมีความไวต่อเชื้อต่ำ

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