Virulences of <I>Beauveria bassiana</I> and <I>Metarhizium anisopliae</I> for Controlling <I>Phenacoccus manihoti</I> Matile-Ferrero and <I>Planococcus lilacinus</I> (Cockerell)
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Abstract
Beauveria bassiana and Metarhizium anisopliae are the entomopathogenic fungi of various insect pests. The objective of this study was to evaluated the virulence of two isolates of B. bassiana isolated from aphids (BA) and brown planthopper (BN) and one isolate of M. Anisopliae (M001) isolated from sugarcane longhorn stem borer for virulence tests against Phenacoccus manihoti Matile-Ferrero and Planococcus lilacinus (Cockerell). The experiments were conducted in laboratory including of five levels of conidia suspensions at 1 × 104, 1 × 105, 1 × 106, 1 × 107 and 1 × 108 conidia/ml. with four replications and infected insects were checked every day up to 7 days after application. The results found that B. bassiana (BA), B. bassiana (BN) and M. anisopliae (M001) showed the highest percent mortality to P. manihoti at 1 × 108 conidia/ml. The average percent mortalities were 75.83 ± 2.89, 65.83 ± 7.22 and 55.00 ± 6.61 percent, respectively while B. bassiana (BA), B. bassiana (BN) and M. anisopliae (M001) showed the highest percent mortality to P. lilacinus at 1x108 conidia / ml and the average percent mortalities were 74.17 ± 7.64, 70.83 ± 3.82 and 61.67 ± 7.22 percent, respectively within 7 days. LC50 of B. bassiana (BA), B. bassiana (BN) and M. anisopliae (M001) on P. manihoti were 5.69 × 105, 1.34 × 106 and 4.29 × 106 conidia/ml, and those on P. lilacinus were 1.34 × 106, 9.37 × 105 and 1.65 × 106 conidia/ml, respectively.
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