Selection of Rhizobacteria Against of Gummy Stem Blight Pathogen of Melon
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Abstract
Selection of rhizobacteria from soil cultivated with melon crops from Pa Sang district, Lamphun province and Mae Hia Agricultural Research, Demonstrative and Training Center, Faculty of Agriculture, Chiang Mai University for controlling Didymella bryoniae causal agent of melon gummy stem blight disease was conducted. Total of 149 rhizobacteria isolates were obtained and used for evaluation of antagonistic activity against D. bryoniae by dual culture method. Nine isolates of rhizobacteria showed the growth inhibition of D. bryoniae. Among them, isolate S93 and S138 showed the highest antagonistic activity to inhibit mycelial growth with 81.20 and 82.40%, respectively. Moreover, cell-free culture filtrated of isolates S93 caused malformation and swelling germtube of fungal pathogen and isolate S138 could delay fungal spore germination. Two antagonistic rhizobacteria were identified based on morphological characteristic and 16S rDNA gene. The result showed that isolate S93 was gram positive bacterium with rod shape cell and the 16S rDNA sequence of S93 showed 99% homology with Bacillus subtilis. Meanwhile, isolate S138 was gram negative bacterium with rod shape cell and its partial sequence was 99% homology with Enterobacter asburiae.
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