Effects of Algal Strains and Culture Media on Lipid Productivity in Green Microalgae, Genus <I>Chlorella</I>
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Abstract
The effects of microalgae strains, culture media and interaction of microalgae strain and culture media were investigated on their specific growth rate, biomass yield, biomass productivity, lipid content and lipid productivity. Three strains of microalgae named 6-4, 208 and 209 were enriched in three different growth media (TAP, BG11 and N8 medium). The result showed that microalgae strain had statistically significant effect on lipid content and productivity. Microalgae strain 6-4 showed the highest lipid content 8.33% and lipid productivity 1.39 g/L/d. Culture media had significant effect on all parameter (specific growth rate, biomass, biomass productivity, lipid content and lipid productivity). TAP culture medium showed the highest specific growth rate, biomass yield and biomass productivity at 0.17 /d, 1.11 g/L and 0.12 g/L/d, respectively. However, the lipid content and lipid productivity from microalgae culturing by TAP and BG11 were not statistically significant difference. The interaction of microalgae strain and culture media were statistically significant effect on specific growth rate, lipid content and lipid productivity. Microalgae strain 6-4 culturing in TAP media showed the highest lipid content 10.55% and lipid productivity 1.76 g/L/d. Due to the fact that lipid productivity is a key factor for biofuel production therefore microalgae strain 6-4 culturing in TAP medium should be considered.
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ผกามาศ ชิดเชื้อ ปติรุจ จิรกาลวงศ์ และ อรอุมา ตนะดุลย์. 2560. วิธีการทำให้เซลล์แตกเพื่อการสกัดน้ำมันจากสาหร่ายขนาดเล็ก. วารสารเกษตร 33(2): 185-191.
Chisti, Y. 2007. Biodiesel from microalgae. Biotechnology Advances 25(3): 294-306.
Gour, R.S., A. Kant and R.S. Chauhan. 2014. Screening of microalgae for growth and lipid accumulation properties. Journal of Algal Biomass Utilization 5(1): 38-46.
Griffiths, M.J. and S.T.L. Harrison. 2009. Lipid productivity as a key characteristic for choosing algal species for biodiesel production. Journal of Applied Phycology 21(5): 493-507.
Hamedi, S., M.A. Mahdavi and R. Gheshlaghi. 2012. Lipid content and biomass production of Chlorella vulgaris is affected by growth conditions. pp. 65-68. In: Proceeding of the Second Iranian Conference on Renewable Energy and Distributed Generation. IEEE, Tehran.
Harris, E.H. 1989. The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego, 780 p.
Leu, J.Y and Y.H. Lin. 2013. Optimization of nutritional compositions of growth medium for Chlorella sp. FJ3 growth kinetics in batch and continuous-flow photoreactors. Environmental Technology 34(20): 2841-2851.
Mandalam, R.K. and B.O. Palsson. 1998. Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures. Biotechnology and Bioengineering 59(5): 605-611.
Mata, T.M., A.A. Martins and N.S. Caetano. 2010. Microalgae for biodiesel production and other applications: a review. Renewable and Sustainable Energy Reviews 14(1): 217-232.
Miazek, K., D. Goffin, A. Richel and C. Remacle. 2014. Growth of Chlorella in the presence of organic carbon: a photobioreactor study. In: Proceeding of International Procesni Technika, Prague, Czech Republic, pp. 5-10.
Pittman, J.K., A.P. Dean and O. Osundeko. 2011. The potential of sustainable algal biofuel production using wastewater resources. Bioresource Technology 102(1): 17-25.
Sharma, A.K., P.K. Sahoo, S. Singhal and A. Patel. 2016. Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp. 3 Biotech 6(2): 116. doi: 10.1007/s13205-016-0434-6.
Stanier, R.Y., R. Kunisawa, M. Mandel and G. Cohen-Bazire. 1971. Purification and properties of unicellular blue-green algae (Order Chroococcales). Bacteriological Reviews 35(2): 171-205.
Tanadul, O., J.S. van der Gheynst, D.M. Beckles, A.L.T. Powell and J.M. Labavitch. 2014. The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock. Biotechnology and Bioengineering 111(7): 1323-1331.
Wang, W., F. Han, Y. Li, Y. Wu, J. Wang, R. Pan and G. Shen. 2014. Medium screening and optimization for photoautotrophic culture of Chlorella pyrenoidosa with high lipid productivity indoors and outdoors. Bioresource Technology 170: 395-403.