Association Between <I>cGH</I> and <I>IGF-1</I> Genotypes Patterns and Body Weight and Growth of Khai Mook Esarn 2 Chicken
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Abstract
Chicken growth hormone (cGH) and insulin-like growth factor (IGF-I) have a crucial role for regulating of growth in chickens, which improvements of genetic variation of growth might result in an increase of productive performance in chickens. The objective of this study was to investigate genetic variation in cGH and IGF-1 gene and evaluate associations between genotypes of these genes, and body weight and growth traits of Khai Mook Esarn 2 (KME2) chickens. A total of 318 chickens were collected from the 5th generation of KME2. Genetic variation of cGH and IGF-1 genes was genotyped by PCR-RFLP method, and an association analysis was performed by Proc GLM. In the study population, the genotypic frequency of cGH/EcoRV by SNP region in Chr.27 (G>A +1705 Intron3) was 0.101 for AA, 0.550 for AG and 0.349 for GG. While the genotype frequency of the IGF-1/Hinfl by SNP region in Chr.1 (A>C 5’UTR) was 0.160 for AA, 0.591 for AC and 0.248 for CC. The association analysis revealed association between variations of IGF-1/Hinfl and body weight in KME2. The AC genotypic group had the highest birth weight (41.39 ± 0.44 g) compared to those in the AA (39.70 ± 0.51 g) and CC (40.49 ± 0.29 g) (P<0.05). In addition, the AC genotype tended to have the highest body weight at 10 weeks (P<0.1), but there was no association between IGF-1/Hinfl genotypes and the average daily gain at 4 weeks, breast width and breast circumference. Meanwhile, there was no association among genotypes of cGH/EcoRV and body weight, average daily gain, breast width and breast circumference (P>0.05) of KME2. These findings suggested that variation in IGF-1/Hinfl could potentially be useful as genetic marker for improving body weight of KME2 chickens.
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