Effects of Edible Insects as Ingredient in Sabouraud Dextrose Broth and Culture Media on Growth of Gold Cordyceps, <I>Cordyceps militaris</I> (L.) Link
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Abstract
Gold cordyceps (Cordyceps militaris (L.) Link) contained of cordycepin and adenosine as bioactive compounds, which described as a medicine and functional food. It can grow on several insect species, especially edible insects in order Lepidoptera, Orthoptera and Coleoptera. The objective was to study the effect of edible insects as an ingredient in Sabouraud dextrose broth (SDB) and the culture media on the growth and the bioactive compound of C. militaris. The four tested edible insect species were pre-pupa and pupa of silkworm (Bombyx mori), adult cricket (Gryllus bimaculatus), bamboo caterpillar (Omphisa fuscidentalis) and mealworm (Tenebrio molitor). The result revealed that the SDB mixed with cricket yielded the highest viability of 3.5 x 103 cfu/ml. Then, the media were blended with two kinds of media, Riceberry rice + all tested edible insects and Khao Dawk Mali 105 (KDML 105) rice + all tested edible insects. It was obvious that C. militaris culturing on KDML105 rice + silkworm pupa gave the maximum fruiting body length of 3.14 cm whereas the average highest fresh and dry weights, 5.56 and 1.17 g, obtained from Riceberry rice + silkworm pre-pupa medium. The content of bioactive compounds, cordycepin and adenosine, in the fruiting bodies and the media were analyzed. The highest cordycepin content (1,266.74 mg/100 g) was found in fruiting body culturing on KDML105 rice + cricket medium while the highest adenosine content (94.91 mg/100 g) was found in KDML105 rice + cricket medium. The result of this study will be a guideline to develop the technique of gold cordyceps culture for farmers and researchers in the future.
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References
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