Screening and Efficiency Assessment of Rhizobacteria for Growth Enhancement of Rice Seedlings
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Abstract
Rice (Oryza sativa L.) is one the most important economic crop of Thailand and high input of chemical fertilizers is a common practice for rice production. This practice has given rise to several problems such as contamination of water, degradation of soil and loss of biodiversity ultimately leading to health risks for humans. The use of beneficial bacteria is an alternative to reduce the mention problems. Therefore, in the present study, rhizobacteria were isolated from 4 organic rice locations in Chiang Mai province i.e. Mae Wang district (2 sites), Mae Taeng district (1 site) and Phrao district (1 site). A total of 56 isolates of rhizobacteria was obtained. After preliminary qualitative-screening on agar plate, only 13 isolates showed promising abilities in nitrogen fixing and phosphate solubilizing abilities. The results of quantitative-screening in broth culture indicated that isolate RMW4NF1 showed highest ability in nitrogen fixation followed by RMT2NF4 (887.4 and 822.9 nmol C2H4/tube/24hr, respectively), and RMW4NF1 exhibited highest ability in phosphate solubilization with value of 181.46 mgP/L. Evaluation of indole-3-acetic acid (IAA) indicated that RMW3NF4 could produce highest amount of IAA followed by RMT2NF4 (34.93 and 31.05 mg IAA/L, respectively). From all the evaluations of plant growth promoting potential, six isolates i.e. RMW2Egg2, RMW2Egg8, RMW4Egg5, RMW4NF1, RMT2Egg2 and RMT2NF4 were selected to test their effectiveness on growth and nutrients uptake of rice seedlings. The results showed that the application of almost all the isolates gave significantly higher growth and nutrients uptake than the control. Isolate RMT2NF4 gave 214 and 74% higher nitrogen and phosphorus uptake than the control, respectively. Therefore, this isolate has a high potential to be developed as biofertilizer for rice.
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References
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