Crossability and <I>In Vitro</I> Seed Germination of Some Terrestrial Orchid Species of Genus <I>Habenaria</I> and <I>Pecteilis</I>
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Abstract
Habenaria and Pecteilis are terrestrial orchids in subfamily Orchidoideae. Flowers are various in colors and shapes, have long blooming period and some species have fragrance. All of these characteristics are suitable for breeding program. This research was conducted to study crossability of nine species, H. dentata, H. erichmichaelii, H. lucida, H. marginata, H. myriotricha, H. rhodocheila (orange and red color), H. rostellifera, P. hawkesiana and P. susannae. They were employed for selfed, interspecific hybridization and intergeneric hybridization. It was found that number of fruit set of selfed was 60-100%, H. dentata had the greatest seed viable at 55.80%. Number of fruit set of interspecific hybridization was 0-100%, H. marginata × H. rostellifera had the greatest seed viable at 92.60%. Number of fruit set of intergeneric hybridization was 0-100%, P. hawkesiana × H. myriotricha had the greatest seed viable at 65.43%. In vitro seed culture was done using MS basal medium for seed germination. It was found that seed of selfed H. erichmichaelii could germinate within 99 days at 36.60% germination. Interspecific hybridization seed of H. erichmichaelii × H. rhodocheila (red) had the greatest germination at 98.00% within 154 days and intergeneric hybridization seed of H. dentata × P. hawkesiana had the greatest germination at 50.00% within 95 days. Species that were suitable for maternal plant for interspecific crosses were H. erichmichaelii and H. dentata.
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References
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