Effect of Culture Media, Plant Growth Regulators and Types of Explant on Proliferation of the Genus Mammillaria and Ferocactus ผลของสูตรอาหาร สารควบคุมการเจริญเติบโต และชนิดของชิ้นส่วนพืชต่อการเพิ่มปริมาณกระบองเพชรสกุลแมมมิลลาเรียและเฟโรแคคตัส

Abstract

In vitro cactus propagation of the genus Mamillaria and Ferocactus were to study types of explant and culture media on plant growth of this objective studies. The explants (0.5 mm size) of two genus cactus were cut and uncut longingly with a sharp sterilized surgical blade and cultured on MS (Murashige and Skoog), ½MS and ¼MS medium. After 4 weeks of culture, the result showed that the plantlet of Mammillaria plumose Pink Flower (0.5 mm size) was uncut longingly with a sharp sterilized surgical blade and the plantlet of M. microthele (0.5 mm size) was cut longingly with a sharp sterilized surgical blade cultured on MS, ½MS and ¼MS medium gave the highest survival rate at 100%. The plantlet of M. microthele (0.5 mm size) was uncut longingly with a sharp sterilized surgical blade cultured on ½MS and ¼MS medium gave the highest survival rate at 100%. While the plantlet of Ferocactus cylindraceus (0.5 mm size) was cut longingly with a sharp sterilized surgical blade cultured on MS and ¼MS medium and the plantlet of F. cylindraceus (0.5 mm size) was uncut longingly with a sharp sterilized surgical blade cultured on MS and ¼MS gave the highest survival rate at 100%. After 12 weeks of culture, the result showed that M. plumosa Pink Flower was cut and uncut longingly with a sharp sterilized surgical blade cultured on ½MS medium gave the highest plant growth. While M. microthele was cut and uncut longingly with a sharp sterilized surgical blade cultured on ¼MS and MS medium gave the highest plant growth. For F. cylindraceus was cut and uncut longingly with a sharp sterilized surgical blade cultured on ½MS and MS medium gave the highest plant growth. For effect of various concentration of BA (N₆-Benzyladenine) on shoot induction and cactus proliferation. The callus (0.5 g) of M. plumose Pink Flower and M. microthele were cultured on MS medium supplemented with 0.0, 0.5 and 1.0 mg/L. After 4 weeks of culture, the result showed that M. Plumose Pink Flower and M. microthele were cultured on MS medium supplemented with 1 mg/L BA gave the highest of fresh weight at 4.44 g and 5.15 g, respectively. While M. plumosa Pink Flower and M. microthele were cultured on MS medium supplemented with 0.5 mg/L BA gave the highest shoot formation at 1.56 shoots/explant.