Effect of Plant Spacing on Growth, Yield, and Cannabidiol Content of Outdoor-Grown Hemp (Cannabis sativa L.)

Authors

  • Watcharin Jantawan Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand https://orcid.org/0009-0001-2632-6709
  • Orapin Saritnum Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
  • Ravee Ganeshaborirak Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
  • Seksan Songchainthuek The Office of Agricultural Research and Extension, Maejo University, Chiang Mai, Thailand
  • Winai Wiriya-Alongkorn Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand

DOI:

https://doi.org/10.14456/jare-mju.2026.1

Keywords:

plant spacing, hemp, cannabidiol, factor, variety

Abstract

Since the legalization of the cultivation, utilization, and research of hemp and cannabis plants in Thailand in 2022, commercial cultivation of these crops has significantly increased. However, such enterprises have experienced varying outcomes, including both successful ventures and substantial financial losses, mainly due to insufficient knowledge, especially regarding optimal plant spacing and appropriate varieties for open-field cultivation. This study was conducted at the Hemp Research and Development Center, Maejo University Chiang Mai, Thailand. The experiment followed a 3×3 factorial arrangement in a randomized complete block design (RCBD), with factor 1 being plant spacing (1×1 m, 2×1 m, and 2×2 m), and factor 2 being hemp variety (EHFGP#1, EHFGP#3, and EHFGP#8). The results showed that plant spacing had a significant effect on plant height, plant canopy diameter, number of nodes per plant, number of branches per plant, inflorescence size, fresh flower yield per plant, dry flower yield per plant, and dry flower yield per rai (1,600 m²). Interaction effects indicated that the EHFGP#3 variety, when planted at 1×1 m spacing, had the highest average plant height at 238.38 cm. EHFGP#1, when planted at 2×1 m and 2×2 m spacing, produced the highest number of nodes and branches per plant, with statistically significant differences. Inflorescence size varied with both variety and plant spacing. A 2×2 m spacing resulted in the largest inflorescence size for EHFGP#3 and EHFGP#8, while a 2×1 m spacing produced the largest inflorescence size for EHFGP#1. In terms of cannabidiol (CBD) content, cultivar was a highly significant factor. Cultivars EHFGP#1 and EHFGP#3 exhibited the highest CBD concentrations, ranging from 11.76–12.93% and 11.79–13.10%, respectively. The interaction between cultivar and plant spacing was significant; however, plant spacing alone had no effect on CBD content.

References

Burczyk, H., L. Grabowska, M. Strybe and W. Konczewicz. 2009. Effect of sowing density and date of harvest on yields of industrial hemp. Journal of Natural Fibers 6: 204–218.

Chen, X., H. Deng, J.A. Heise, D.P. Puthoff, N. Bou-Abboud, H. Yu and J. Peng. 2021. Contents of cannabinoids in hemp varieties grown in Maryland. ACS Omega 6: 32186–32197.

Darby, H., R. Malone, J. Bruce, I. Luke and S. Ziegler. 2020. 2019 Hemp Cannabidiol Plant Spacing x Planting Date Trial. 8 p. In Research Report. Burlington, Vermont: Northwest Crops and Soils Program.

Darby, H., J. Bruce, I. Krezinski, and L. Ruhl. 2021. 2020 Hemp Flower Variety Trial. 20 p. In Research Report. Burlington, Vermont: Northwest Crops and Soils Program.

Darby, H., J. Bruce, I. Krezinski, R. Malone and S. Ziegler. 2022. 2020 Hemp Flower Plant Spacing x Planting Date Trial. 8 p. In Research Report. Burlington, Vermont: Northwest Crops and Soils Program.

Department of Agriculture (Thailand). 2022. Notification of the Department of Agriculture Re: Public Announcement of the Application for the Issuance of a Certificate of Registered Plant Variety Pursuant to the Plant Varieties Act, B.E. 2518 (1975). Bangkok: Department of Agriculture, Ministry of Agriculture and Cooperatives. 57 p. [in Thai]

Department of Medical Services (Thailand). 2024. Guidance on Cannabis for Medical Use. 6th Revised Edition. Bangkok: Department of Medical Services, Ministry of Public Health. 57 p. [in Thai]

Duangdamrong, J., K. Jamparngernthaweesri, Y. Sathiyos, S. Poopong, N. Lumlerdkij and P. Akarasereenont. 2022. Evidence of cannabis for medical uses and its indications: an evidence-based comparison between Thai traditional medicine and western medicine. Siriraj Medical Bulletin 15(3): 155–163. [in Thai]

García-Tejero, I.F., V.H. Durán-Zuazo, R. Pérez-Álvarez, A. Hernández, S. Casano, M. Morón and J.L. Muriel-Fernández. 2014. Impact of plant density and irrigation on yield of hemp (Cannabis sativa L.) in a Mediterranean semi-arid environment. Journal of Agricultural Science and Technology 16: 887–895.

García-Tejero, I.F., A. Hernández, C. Ferreiro-Vera,V., H. Durán Zuazo, J. Hidalgo García, C. Sánchez-Carnerero and S. Casano. 2020. Yield of new hemp varieties for medical purposes under semi-arid Mediterranean environment conditions. Comunicata Scientiae 11: e3264.

Koren, J. 2007. Influence of plantation row spacing on quality and yield of hops. Plant, Soil and Environment 53(6): 276–282.

Linder, E.R., S. Young, X. Li, S. Henriquez Inoa and D.H. Suchoff, 2022. The effect of transplant date and plant spacing on biomass production for floral hemp (Cannabis sativa L.). Agronomy 12: 1856.

Ministry of Public Health (Thailand). 2022. Ministerial Notification of the Ministry of Public Health Re: Specifying Narcotic Substances in Category 5, B.E. 2565 (2022). The Royal Thai Government Gazette 139 (Special Part 35 Ngor): 8. [in Thai]

O'Brien, K. and P. Blair. 2021. Medicinal Cannabis and CBD in Mental Healthcare. Cham: Springer. 361 p.

Office of Industrial Economics. 2022. Action plan for the development of the industrial hemp industry towards commercialization (2023-2027). Retrieved from http://bioie.oie.go.th/oieqrcode/uploadFile/oie736117653_2029136235.pdf [in Thai]

Pinmanee, S., R. Yanaphan, S. Kuppatarat, P. Akaranuchat, S. Kaewchumchuem, P. Meepanya, W. Pansupa and S. Pripanachaiman. 2013. Research and Development on Cultivation and Processing of Products from Hemp: A Final Report of Action Research in the Royal Project Foundation Areas. 56 p. In Research Report. Chiang Mai: Highland Research and Development Institute (Public Organization). [in Thai]

Visković, J., V.D. Zheljazkov, V. Sikora, J. Noller, D. Latković, C.M. Ocamb and A. Koren. 2023. Industrial Hemp (Cannabis sativa L.) agronomy and utilization: a review. Agronomy 13: 931.

Yadav, S.P.S., N.P. Ghimire and V. Lahutiya. 2023. A comprehensive review of the production technology of Cannabis sativa L. with its current legal status and botanical features. Fundamental and Applied Agriculture 8(1&2): 458–474.

Yanaphan, R. 2016. Hemp Cultivation Manual for Fiber Production under a Controlled System. 1st Ed. Chiang Mai: Highland Research and Development Institute (Public Organization). 28 p. [in Thai]

Yanaphan, R., S. Kuppatarat and S. Pinmanee. 2023. The test of hemp varieties and their potential for fiber production. Khon Kaen Agriculture Journal 51(SUPPL.1): 140–145. [in Thai]

Figure 1  Inflorescence of hemp varieties; (A, D) EHFGP#1, (B, E) EHFGP#3 and (C, F) EHFGP#8

Published

2026-03-19

How to Cite

Jantawan, W., Saritnum, O. ., Ganeshaborirak, R. ., Songchainthuek, S. ., & Wiriya-Alongkorn, W. . (2026). Effect of Plant Spacing on Growth, Yield, and Cannabidiol Content of Outdoor-Grown Hemp (Cannabis sativa L.). Journal of Agricultural Research and Extension, 43(1), 1–14. https://doi.org/10.14456/jare-mju.2026.1

Issue

Section

Research Article