Effect of rice meal media in combination with the protective agent on cell protection of probiotic strains Lactobacillus acidophilus TISTR 1338 for animal feed industry

Abstract

The feed industry has recognized the importance of producing probiotics for animal feed. However, due to product preservation problems due to the inability to keep probiotic microorganisms alive in food for a long time at room temperature, the idea was to add cell protection agents to help protect probiotic microorganisms. in animal feed products by using rice as a medium in combination with cell protection agents. In this test, media from rice meal were used together with pH adjustments at 5.5, 6.0, 6.5 and 7.0, different concentrations of glycerol cell envelopes 5, 10, 15 and 20 % and different concentrations of maltodextrin cell protection were used at 20, 30, 40 and 50 %. The pH of 7.0 was able to sustain probiotic cells in milled rice for up to 19 days at room temperature, with the probiotic strain Lactobacillus acidophilus TISTR 1338 remaining at 3.47 log CFU/g at 3.47 log CFU/g. The addition of glycerol at a 15 % concentration in combination with a pH adjustment of 7.0 was able to protect probiotic cells for only 10 days with the remaining cell survival of 3.73 × 10⁴ CFU/g.