Effects of Multi-Strain Bacillus Species Supplementation in Drinking Water on Cecal Microbiology, Apparent Nutrient Digestibility, and Small Intestinal Morphology of Broilers
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Abstract
The current ban on the usage of antibiotics as a feed additive forced for alternative sources of antibiotics in the feed industry. Presently, probiotics are measured as a potential substitute for antibiotic as a live biotherapeutic mediator. Accordingly, this study was concentrated on estimating the effect of multi-strain Bacillus species (Bacillus spp.) on cecal microbiology, apparent nutrient digestibility, and small intestinal morphology of broiler chickens. A total of three hundred 1-d-old broilers (Ross 308) were randomized into 3 groups, each group with 4 replications (n = 10). The treatments were, Bacillus spp. supplementation in drinking water with 0 (control), 1, and 2 g/l. During 21 day of experiment, diets and drinking water were offered ad libitum to the birds. The results of the Bacillus spp. supplementation groups revealed a significant improvement in nutrient digestibility of dry matter (linear, P<0.05), and affected to ether extract and crude fiber digestibility (quadratic, P<0.01). Also, lactic acid bacteria (Lactobacillus sp. and Bifidobacterium sp.) (quadratic, P<0.01) and Enterococcus spp. (linear, P<0.05) in cecum increased in the Bacillus spp. supplementation groups. However, a number of microber in cecum and apparent nutrient digestibility were not different between 1 g/l and 2 g/l supplementation groups (P>0.05). The supple mentation of Bacillus spp. did not affect small intestinal morphology (P>0.05). This study showed that multi-strain Bacillus species has the beneficial effect on nutrient digestibility and improved microbial ecology of broiler chickens fed 1 and 2 g/l of Bacillus spp. in drinking water.
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