Efficiency of maize (Zea mays L.) breeding methods of doubled haploid, modified single hill and pedigree breeding for developing inbred lines and hybrids

Abstract

There are various breeding methods for developing inbred lines and hybrids in maize. The objective of this study was to compared four methods for developing inbred lines including doubled haploid in first generation (DHS0) and doubled haploid in second generation (DHS1), modified single hill (MSH) and pedigree (PED) methods to use in the development of high yield inbred lines and high yield hybrid maize. The CPQ-1302 variety was used to develop inbred lines by using those 4 breeding methods. After that, the obtained inbred lines were cultivated for yield comparison. Then, the selected inbred lines were used to develop hybrids. The hybrids were cultivated for yield comparison. Experimental design was 14x14 simple lattice square design. The results showed that the doubled haploid in the second generation (DHS1) method was the highest efficient method for developing inbred lines and could generate the highest yield hybrids.
Moreover, this method could develop inbred lines within 3 growing seasons. The subsequent efficient methods were the modified single hill, doubled haploid in first generation (DHS0) and pedigree, respectively. Nonetheless, the usage of Doubled haploid method for development of inbred lines is also limited in some germplasm sources such as low haploid induction rate or double chromosomes technique needs to be developed for increasing the number of inbred lines to generate hybrids.