The comparison of freezing and thawing methods on boar semen quality
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Abstract
The objective of the present study was to compare effects of different freezing and thawing methods on post-thawed boar semen quality. Semen was collected from two different breeds (Pietrain and Duroc). The freezing methods were treatment 1; -35 OC for 5 min and -120 OC for 10 min (11 and 5 cm. above the liquid nitrogen vapor); Treatment 2; -135 OC for 20 min(3 cm. above the liquid nitrogen vapor) and thawing methods were 60 OC for 8 sec; 42 OC for 25 sec) on sperm progressive motility, sperm viability, and plasma membrane integrity were determined.The results revealed that the progressive motility was higher in Pietrain than Duroc (P<0.05). Sperm progressive motility and viability were higher in Treatment 1 compared with Treatment 2 (P<0.05). Semen thawed at at 5 OC for 5 min and 60 OC for 8 sec were higher in every parameters compared to the semen thawed at 42 OC for 25 sec (P<0.05). However there was no interaction effect between freezing and thawing method on post-thawed sperm quality. In conclusion, it could be inferred that the freezing methods in Treatment 1 (-35 OC for 5 min and -120 OC for 10 min) and thawing methods 5 OC for 5 min could be used to maximize boar cryopreserved semen quality.
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