Isolation of cellulose-degrading bacteria and effective of corncob and water hyacinth decomposition using as substrates

Abstract

The object of this research was to isolate cellulose-degrading bacteria. The experimental design was completely randomized design of three replications. The effective cellulase production was studied observing clear zone on CMC medium. The cellulase activity at 30 and 50°C was estimated using corncob and water hyacinth as substrates. The data analysis was measured by reducing-sugar assay. The results showed that the selected bacteria can produce cellulase at high temperature. The ratio of clear zone width per colony width was significantly difference. The bacteria were identified by 16S rRNA gene as Bacillus sp. strain BDHGL04, Bacillus sp. strain ST-R7 and Alcaligenes sp. BZC5 were 99, 99 and 98% of similarity, respectively. From the test of corncob and the water hyacinth decomposition, the cellulase activity was higher than that of control. Especially, the use of corncob or water hyacinth with Bacillus sp. strain BDHGL04 were 132.44 and 138.37 mg/ml respectively at 50°C. The optical density of bacteria was 0.49 at 600 nm, representing 108 CFU/ml. The both cellulose-degrading bacteria were used to produce an inoculum for accelerated compost decomposition and it was found that the highest decomposition rate of up to 27.22 mg/day at 2 weeks. Hence, the selected bacteria is possible to produce inoculum for
decomposition accelerator of the compost that contains corncob and water hyacinth.