Effect of Semen Preservation Storage at 17 OC between Liquid and Solid Forms combined with Glutathione Supplementation on Boar Semen Quality
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Abstract
The aim of this study was to compare two forms of semen preservation, liquid (absence of gelatin)and solid (presence of 1.5% gelatin (w/v)) combined with different concentrations of glutathione supplementation at 4 levels (0, 0.1, 1 and 5 mM ) on boar semen quality during storage at 17 OC for 10 days. Semen samples were evaluated on Days 1, 4, 7 and 10 after storage. The results showed that after storage on Days 1, 4, 7 and 10, percentages of sperm viability, acrosome integrity, and mitochondrial function of solid semen were significantly higher than liquid semen. However, there was no effect on sperm progressive motility (P>0.05). On Day 10, both forms of sperm preservation combined with glutathione at concentration of 1 and 5 mM had higher sperm progressive motility than 0. 1 and 0 mM groups (P<0.05). On Days 7 and 10 of storage, the highest percentages of sperm viability, acrosome integrity, and mitochondrial function in 1 mM of glutathione was found compared with 0 mM but did not differ with 0.1 and 5 mM of glutathione supplementation (P>0.05). Conclusions, solid semen preservation could increase efficiency of percentages of sperm viability, acrosome integrity, and mitochondrial function more than liquid semen preservation group. The best level of glutathione supplementation combined with both forms of semen preservation was 1 mM.
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References
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