TPS1 and TPS2 gene expression and leaf water potential in two sugarcane varieties under water stress
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Abstract
Water deficit is considered one of the most important factors limiting growth rates and crop productivity. The accumulation of osmoprotectants enables the plants to grow under water stress conditions by maintaining cell water balance. Trehalose-6-phosphate synthase (TPS) genes play crucial roles in trehalose metabolism and involved in abiotic stress tolerance mechanism in plants. To study the physiological response and expression of TPS1 and TPS2 in two sugarcane varieties, the experiments were conducted with KPS 94-13 and KPS 01-11-6 which cultured in vitro and subjected to water deficit stress by addition of 16 % polyethylene glycol (PEG) 6000 in the MS medium for 24, 48, 72, 96 and 120 hrs. Water stress caused the reduction in the total leaf water potential and osmotic potential in both sugarcane varieties. The KPS 94-13 showed significantly less declines in the total leaf water potential than KPS 01-11-6 correlated with the increase in the duration of stress. Expression of TPS1 and TPS2 genes were compared in two sugarcane varieties after treated with16 % PEG 6000 for 24, 48, 72, 96 and 120 hr., using qRT-PCR. The result showed that TPS1 and TPS2 genes expressed in both stress and non-stress conditions. The variability in gene expression profiles in different varieties of sugarcane were detected. The extent of expression of TPS1 and TPS2 genes in KPS 94-13 were observed initially and gradually increased under long-term exposure to stress. In KPS 94-13, the TPS1 and TPS2 expression were up-regulated and showed higher levels than control and KPS 01-11-6.
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