Study efficiency of Cinnamomum cassia essential oils in Coccidiosis prevention caused by E. tenella
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Abstract
Two experiments were conducted to investigate the anti-coccidial property of Cinnamomum cassia essential oil (ES). The in vitro efficiency of C. cassia oil was assessed by the inhibition of sporozoites invasion in MDBK cell lines in experiment 1. The cell lines were contacted with sporozoites from Eimeria tenella in different concentrations of Cassia oil. The correlation between ES concentrations and the percentage of invaded cells was found in logarithmic regression by y =-9.2791ln(x)-31.5858 with the calculated IC50 and IC100values as 0.00016 and 0.03 μl/ml., respectively. Afterwards, three concentrations (0.03, 0.20 and 1.40 μl/ml.) were used to study the optimal usage dose for broilers and found that 0.20 μl/ml. C. cassia oil had an effective anti-coccidial property without negative effect on the water and feed consumption of broilers. Therefore, the efficiency in Coccidiosis prevention of 0.20 μl/ml. C. cassia oil in micro-emulsion product was studied in experiment 2. A total of 768 one-day old broilers were used in a completely randomized design with four treatments and four replicates each (48 birds/replication) and randomly received the following treatments; group 1 was non-challenged with E. tenella and without anti-coccidial product supplementation, group 2-4 were challenged with 2,500 oocysts from E. tenella at 14 day-old and supplemented with 0 and 50 ppm. salinomycin in diet, 0.20 μl./ml. C. cassia oil in micro-emulsion product through drinking water, respectively. Intestinal tissues were collected from 4 birds per each replicate at 21 and 35 day of age for histopathological lesion scoring. Results showed that micro-emulsion product of C. cassia oil had the lower efficiency in Coccidiosis prevention caused by E. tenella than those from in vitro study, probably, due to the side effects of surfactants used in product preparation.
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