Genetic diversity of highland rice landraces from ethnic groups in northern Thailand
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Abstract
Climate of highland agricultural area is not suitable for growing elite rice cultivars. Rice landraces thus play an important role on farmers’ livelihood in mountainous area. The purpose of this study was to evaluate morphological traits and genetic diversity using microsatellite markers in 7 rice landraces varieties totally 28 populations from 4 ethnic groups that grow at the altitudes lower and above 1,000 meters above sea level (MASL). The results showed that highland rice landraces illustrated morphological variation both within population and among populations within variety. Three organelle markers indicated that 84% of rice landraces in this study was Tropical japonica type only 16% was Indica type. Highland rice landraces in this study are genetically variable, and structured associated to subspecies types and the altitudes. This study illustrated morphological variation and genetic diversity including population structure of highland rice landraces of ethnic groups in northern Thailand which was the consequence of local adaptation and traditional agriculture practices. Understanding genetic diversity and population structure can be applied as a guideline for conservation strategy of highland rice landraces involved farmers’ managements and local adaptation for furthers utilization. Keywords: Genetic diversity, Local adaptation, Highland rice landrace, Ethnic groups, Population structure, Subspecies types
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อัครพงค์ อนุพันธ์พงศ์. 2550. บ้านเรือนชาวเขากะเหรี่ยงปกาเกอะญอ : ความยั่งยืนและการปรับตัว ภายใต้นิเวศวัฒนธรรมไร่หมุนเวียน. คณะสถาปัตยกรรมศาสตร์, มหาวิทยาลัยศิลปากร.
Bardenas, E. A., and T. T. Chang, 1965. Morphology and Varietal Characteristics of the Rice Plant, The. Int. Rice Res. Inst.
Bellon, M. R., J. L. Pham, and M. T. Jackson. 1997. Genetic conservation: A role for rice farmers. In N. Maxted, B.V. Ford-Lloyd and J.G. Hawkes (eds.), Plant Conservation: TheIn Situ Approach. London: Chapman and Hall.
Boonsit, P., P. Pongpiachan, S. Julsrigival, and D. Karladee. 2010. Gamma oryzanol content in glutinous purple rice landrace varieties. CMUJ. Nat. Sci. 9, 151-157.
Choudhury, B., M. L. Khan. and S. Dayanandan. 2013. Genetic structure and diversity of indigenous rice (Oryza sativa) varieties in the Eastern Himalayan region of Northeast India. SpringerPlus, 2, 228.
Cruz, N. D., and G. S. Khush, 2000. Rice grain quality evaluation procedures. Aromatic rices, 3, 15-28.Daiponmak, W., P. Theerakulpisut, P. Thanonkao, A. Vanavichit, and P. Prathepha. 2010. Changes of anthocyanin cyanidin-3-glucoside content and antioxidant activity in Thai rice varieties under salinity stress. Sci. Asia. 36:286–291.
Doyle, J.J., and J.L. Doyle. 1987. A rapid DNA isolation Procedure for small quantities of fresh leaf tissue. Focus 12: 13-15.
Earl, D. A. 2012. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour. . 4, 359-361.
Frankel, O.H., A.D.H. Brownand. and J.J. Burdon. 1995. The Conversation of Plant Biodiversity, Cambridge: Cambridge University Press, 299.
Goudet, J. 2001. FSTAT, a program to estimate and test gene diversity and fixation indices (version 2.9. 3). http://www2. unil. ch/popgen/softwares/fstat. htm.
Huang, X., N. Kurata. Z. X. Wang. A. Wang. Q. Zhao. Y. Zhao. and Y. Lu. 2012. A map of rice genome variation reveals the origin of cultivated rice. Nature, 490(7421), 497.
IRRI. 2013. Standard Evaluation System for Rice (SES). International Rice Research Institute, Manila, Philippines
Jamjod, S., N.Yimyam, S. Lordkaew, C. Prom-u-thai, and B. Rerkasem. 2017. Characterization of On-farm Rice Germplasm in an Area of the Crop’s Center of Diversity. CMUJ. Nat. Sci. 16, 85-98.
Juliano, B.O. 2007. Rice chemistry and quality. Philippines: Philrice. p. 402.Liu, K., and S. V. Muse. 2005. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 21, 2128-2129.
Lu, B.R., X. Cai. and J. Xin. 2009. Efficient indica and japonica rice identification based on the InDel molecular method: its implication in rice breeding and evolutionary research. Progress in Natural Science, 19(10), pp.1241-1252.
Maclean, J., B. Hardy, and G. Hettel. 2013. Rice Almanac : Source Book for One of the Most Important Economic Activities on Earth. IRRI. Los Baños, Philippines.
Mulumba, J.W., R. Nankya, J.Adokorach, C. Kiwuka, C. Fadda, P. De Santis, and, D.I. Jarvis. 2012. A risk-minimizing argument for traditional crop varietal diversity use to reduce pest and disease damage in agricultural ecosystems of Uganda. Agriculture, Ecosystems and Environment 157: 70-86.
Okoshi, M., K. Matsuno, K. Okuno, M. Ogawa, T. Itani, and T. Fujimura. 2015. Genetic diversity in Japanese aromatic rice (Oryza sativa L.) as revealed by nuclear and organelle DNA markers. Genet. Resour. Crop Evol. 63: 199-208.
Peakall, R. O. D., and P. E. Smouse. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Resources, 6, 288-295.
Pintasen, S., S. Jamjod, N. Yimyam, and B. Rerkasem, 2007. Variation of grain iron content in a local upland rice germplasm from the village of Huai Tee Cha in northern Thailand. Euphytica, 158(1-2), 27-34.
Prathepha, P., and K. Srisa-ard. 2014. Application of InDel markers for subspecies indica-japonica rice classification in Thai rice germplasm. Genomics and Genetics, 7, 87-96.
Pritchard, J.K., M. Stephens, and P. Donnelly 2000. Inference of population structure using multilocus genotype data. Genetics. 155: 945-959.
Pusadee, T., S. Jamjod, Y.C. Chiang, B. Rerkasem, and B.A. Schaal. 2009. Genetic structure and isolation by distance in a landrace of Thai rice. Proc. Natl. Acad. Sci. U.S.A. 106: 13880-13885.
Pusadee, T., P. Oupkaew, B. Rerkasem, S. Jamjod, and B.A. Schaal. 2014. Natural and human-mediated Selection in a landrace of Thai rice (Oryza sativa). Annals of Applied Biology. 165: 280-292.
Schiller, J. M. 2006. Rice in Laos. Int. Rice Res. Inst..
Shannon, C.E., and W. Weaver. 1949. The Mathematical Theory of Communication. [SI]. Urbana.
Shrestha, R. P., N. Chaweewan. and S. Arunyawat. 2017. Adaptation to Climate Change by Rural Ethnic Communities of Northern Thailand. Climate, 5, 57.
Wang, Y., Y. Wang, X. Sun, Z. Caiji, J. Yang, D. Cui, and L. Han. 2016. Influence of ethnic traditional cultures on genetic diversity of rice landraces under on-farm conservation in southwest China. J Ethnobiol Ethnomed. 12: 51.
Xiong, Z., S. Zhang. Y. Wang. B.V. Ford-Lloyd. M. Tu. X. Jin. Y. Wu. H. Yan. X. Yang. P. Liu. B.R. Lu. 2010. Differentiation and distribution of indica and japonica rice varieties along the altitude gradients in Yunnan province of China as revealed by InDel molecular markers. Genet. Resour. Crop Evol. 57: 891–902.