The use of hydroponic nutrient solutions replacing MS media in Venus Flytrap (Dionaea muscipula) tissue culture

Abstract

enus Flytrap (Dionaea muscipula) is cultivated as an ornamental plant due to its leaf beauty and effective of plumbagin to inhibit bacterial growth. Since natural propagation difficulty, plant tissue culture increasing propagation rate can solve the problem. However the cost of chemicals for plant tissue culture is quite high and not available to the farmers. The objective of this study was to investigate the various concentrations of hydroponic nutrient solution (HNS) on the growth of Venus Flytrap in tissue culture. Four cultivars namely Korean Melody Shark (KMS), Cuppep Trap (CT), Dark Red (DR), Angelwings (AW) were grown on ½MS, ¼MS and HNS (stock A and B at 100x) at 4 and 6 ml/L with Vitamix V 1,000 at 5 and 10 ml/L. The cultures were incubated at temperature 25±2 oC with light 16 hrs./day for 45 days. The results showed that media and cultivars had significant differences (P<0.01) on the number of shoots and plant height. HNS at 6 ml/L with Vitamix V 1,000 at 5 ml/L gave the highest number of shoots (10.00+2.00 shoots) in AW cultivar and also gave the highest plant height (3.40+0.52 cm.) in DR cultivar. As for the root number, the medium and cultivar showed the significant difference (P<0.05) on root number but not on root length (P>0.05). The ¼MS media and AW gave the highest root number (39.00+9.64 roots). In addition, media and cultivars did not show the effect on plant color (P>0.05) [a* and b*]. Therefore, hydroponics nutrient solution at 6 ml/L with Vitamix V 1,000 at 5 ml/L can be used as a replacement for MS media in D. muscipula tissue culture.