Efficiency of Moringa oleifera leaves extract on osteogenic differentiation of porcine bone marrow derived mesenchymal stem cells
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Published:
Apr 26, 2018
Keywords:
Moringa oleifera leaves extract porcine bone marrow derived mesenchymal stem cells osteogenic differentiation
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Abstract
The experiment was conducted to study the efficiency of Moringa oleifera leaf (MOL) extract on osteogenic differentiation of porcine bone marrow derived mesenchymal stem cells (pBM-MSCs). pBM-MSCs were isolated and cultured in completed medium or osteogenic differentiation medium (OstDiff medium) containing 0 (control group), 100, 200, and 300 μg/ml water or ethanolic extract of MOL to explore the osteogenic differentiation ability. The degree of osteogenic differentiation after MOL extract treatment was assessed by alkaline phosphatase (ALP) expression and ALP activity. The results of MOL extract supplementation in osteogenic differentiation demonstrated that pBM-MSCs were increase in all groups of MOL supplementation when compared to the control group (P<0.05). The present study suggested that water and ethanolic extract of MOL promotes osteogenic differentiation of pBM-MSCs.
Article Details
How to Cite
Marupanthorn, K. (2018). Efficiency of Moringa oleifera leaves extract on osteogenic differentiation of porcine bone marrow derived mesenchymal stem cells. Khon Kaen Agriculture Journal, 46(2), 227–236. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/250059
Section
บทความวิจัย (research article)
References
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Anwar, F., Latif, S., Ashraf, M., Gilani, and A.H. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res. 21(1): 17-25.
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Bruckner, S., H.M. Tautenhahn, S. Winkler, P. Stock, M. Dollinger, and B. Christ. 2014. A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research. Exp Cell Res. 321(2): 267-75.
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Onu, P.N., and A.O. Aniebo. 2011. Influence of Moringa oleifera leaf meal on the performance and blood chemistry of starter broilers. International Journal of Food, Agriculture and Veterinary Sciences. 1(1): 38-44.
Ratsada, P., S. Maitree, C. Suphachai, T. Rungthip, K. Paween, J. Kwanchaya, and V. Pramote. 2016. Cytotoxicity evaluation of leaf-extract from Moringa oleifera, Cratoxylum formosum, and Mangifera indica. Naresuan Phayao Journal. 9(2): 5-8.
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Sreelatha, S., and P. R. Padma. 2009. Antioxidant Activity and Total Phenolic Content of Moringa oleifera Leaves in Two Stages of Maturity. Plant Foods Hum Nutr. 64: 303–311.
Stenderup, K., J. Justesen, C. Clausen, and M. Kassem. 2003. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone. 33(6): 919-26.
Vongsaka, S., P. Sithisarna, S. Mangmool, S. Thongpraditchote, Y. Wongkrajang, and W. Gritsanapan. 2013. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Industrial Crops and Products. 44: 566–571.
Wan, D.C., M.T. Siedhoff, M.D. Kwan, R.P. Nacamuli, B.M. Wu, and M.T. Longaker. 2007. Refining retinoic acid stimulation for osteogenic differentiation of murine adipose-derived adult stromal cells. Tissue engineering. 13(7): 1623-31.
Alikwe, P.C.N., and M.S. Omotosho. 2013. An evaluation of the proximate and phytochemical composition of Moringa oleifera leaf meal as potential feedstuff for non-ruminant livestock. AgroSearch. 13: 17-27.
Anwar, F., Latif, S., Ashraf, M., Gilani, and A.H. 2007. Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res. 21(1): 17-25.
Bharti, D., S.B. Shivakumar, R.B. Subbarao, and G.J. Rho. 2016. Research advancements in porcine derived mesenchymal stem cells. Current Stem Cell Research & Therapy. 11(1): 78–93.
Bruckner, S., H.M. Tautenhahn, S. Winkler, P. Stock, M. Dollinger, and B. Christ. 2014. A fat option for the pig: hepatocytic differentiated mesenchymal stem cells for translational research. Exp Cell Res. 321(2): 267-75.
Chirag, P., M. A. Rangrez, and P. Pragna. 2015. Studies on the Osteoprotective and Antidiabetic Activities of Moringa Oleifera Plant Extract. IOSR Journal of Pharmacy. 5(5): 19-22.
Deans, R.J., and A.B. Moseley. 2000. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol. 28: 875-884.
Friedenstein, A.J, R.K, Chailakhyan, N.V. Latsinik, A.F. Panasyuk, and I.V. Keiliss-Borok. 1974. Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo. Transplantation. 17: 331–340.
Juhasova, J., S. Juhas, J. Klima, J. Strnadel, M. Holubova, and J. Motlik. 2011. Osteogenic differentiation of miniature pig mesenchymal stem cells in 2D and 3D environment. Physiol Res. 60(3): 559-71.
Jung, I. L. 2014. Soluble Extract from Moringa oleifera Leaves with a New Anticancer Activity. PLoS ONE. 9(4): e95492.
Kapinas, K., and A.M. Delany biogenesis and regulation of bone remodeling. Arthritis Res Ther. 13(3): 220.
Kulisara, M., and K. Wisit. 2016. The Effects of Moringa Oleifera Lam. Leaves Extract on Osteogenic Differentiation of Porcine Bone Marrow Derived Mesenchymal Stem Cells. In: 4th International Conference on Advances in Agricultural, Biological & Ecological Sciences (AABES-16) Dec. 1-2, 2016, London.
Le Blanc, K., and O. Ringden. 2006. Mesenchymal stem cells: properties and role in clinical bone marrow transplantation. Curr Opin Immunol. 18: 586-91.
Nakashima, K., and B. de Crombrugghe. 2003. Transcriptional mechanisms in osteoblast differentiation and bone formation. Trends Genet. 19(8): 458-66.
Onu, P.N., and A.O. Aniebo. 2011. Influence of Moringa oleifera leaf meal on the performance and blood chemistry of starter broilers. International Journal of Food, Agriculture and Veterinary Sciences. 1(1): 38-44.
Ratsada, P., S. Maitree, C. Suphachai, T. Rungthip, K. Paween, J. Kwanchaya, and V. Pramote. 2016. Cytotoxicity evaluation of leaf-extract from Moringa oleifera, Cratoxylum formosum, and Mangifera indica. Naresuan Phayao Journal. 9(2): 5-8.
Rho, G.J., B.M. Kumar, and S.S. Balasubramanian. 2009. Porcine mesenchymal stem cells-current technological status and future perspective. Front Biosci (Landmark Ed). 1(14): 3942-61.
Sanchez-Ramos, J., S. Song, F. Cardozo-Pelaez, C. Hazzi, T. Stedeford, A. Willing, et al. 2000. Adult bone marrow stromal cells differentiate into neural cells in vitro. Experimental neurology. 164(2): 247-56.
SAS. 2006. STAT User’s Guide Release 9.1.3. NC: SAS Inst., Cary.
Singh, V., N. Singh, U. S. Pal, S. Dhasmana, S. Mohammad, and N. Singh. 2011. Clinical evaluation of cissus quadrangularis and Moringa Oleifera and osteoseal as osteogenic agents in mandibular fracture. National Journal of Maxillofacial Surgery. 2(2): 132-136.
Sreelatha, S., and P. R. Padma. 2009. Antioxidant Activity and Total Phenolic Content of Moringa oleifera Leaves in Two Stages of Maturity. Plant Foods Hum Nutr. 64: 303–311.
Stenderup, K., J. Justesen, C. Clausen, and M. Kassem. 2003. Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells. Bone. 33(6): 919-26.
Vongsaka, S., P. Sithisarna, S. Mangmool, S. Thongpraditchote, Y. Wongkrajang, and W. Gritsanapan. 2013. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Industrial Crops and Products. 44: 566–571.
Wan, D.C., M.T. Siedhoff, M.D. Kwan, R.P. Nacamuli, B.M. Wu, and M.T. Longaker. 2007. Refining retinoic acid stimulation for osteogenic differentiation of murine adipose-derived adult stromal cells. Tissue engineering. 13(7): 1623-31.
