Enhancing lipid productivity in microalgae by mutation induction
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Abstract
The aim of this study was to examine the ability of herbicide quizalofop on increasing the intracellular lipid accumulation in microalgae. Chlorella sp. was cultured in liquid media containing quizalofop at the start concentration of 1 μM. The survival cells were selected and continue cultured in 2.5, 5, 7.5, 10, 12.5 and 15 μM quizalofop-containing media (respectively). After seven rounds of mutation-selection, three mutagenized treatments at 5, 7.5 and 10 μM quizalofop were selected and named as M5, M7.5 and M10, respectively. The selected microalgae were cultured for determine the specific growth rate, biomass yield, biomass productivity, lipid content and lipid productivity compared with the wild type. The results showed that mutagenized microalgae M10 yielded the highest lipid content 0.9% and lipid productivity 0.15 g/L/d which were significantly higher than the wild type up to 34.33 and 36.36%, respectively. This study highlights the ability to enhance intracellular lipid accumulation in microalgae using random mutagenesis by herbicide. This approach can be used to improve lipid productivity in microalgae as potential feedstock for biodiesel production in future breeding programs.
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ผกามาศ ชิดเชื้อ, ปติรุจ จิรกาลวงศ์ และอรอุมา ตนะดุลย์. 2560. วิธีการทำให้เซลล์แตกเพื่อการสกัดน้ำมันจากสาหร่ายขนาดเล็ก. วารสารเกษตร. 33(2): 185-191.
American Museum of Natural History. 2013. Optimizing Algae Biofuels: applied natural selection to improve lipid synthesis. Available: https://goo.gl/c5tRu7. Accessed Sep. 15, 2017.
Chisti, Y. 2007. Biodiesel from microalgae. Biotechnol. Adv. 25(3): 294-306.
Doan, T.T.Y. and J.P. Obbard. 2012. Enhanced intracellular lipid in Nannochloropsis sp. via random mutagenesis and flow cytometric cell sorting. Algal Res. 1: 17–21.
Lee, B., G. Choi, Y. Choi, M. Sung, M.S. Park, and J. Yang. 2014. Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii. Korean J. Chem. Eng. 31(6): 1036-1042.
Lim, D.K.Y., and P.M. Schenk. 2017. Microalgae selection and improvement as oilcrops: GM vs non-GM strain engineering. AIMS Bioengineering. 4(1): 151-161.
Ma, Y., Z. Wang, M. Zhu, C. Yu, Y. Cao, D. Zhang, and G. Zhou. 2013. Increased lipid productivity and TAG content in Nannochloropsis by heavy-ion irradiation mutagenesis. Bioresour. Technol. 136: 360–367.
Mehtani, J., N. Arora, A. Patel, P. Jain, P.A. Pruthi, K.M. Poluri, and V. Pruthi. 2017. Augmented lipid accumulation in ethyl methyl sulphonate mutants of oleaginous microalgae for biodiesel production. Bioresour. Technol. 242: 121-127.
Patel, V.K., D. Maji, S.S. Pandey, P.K. Rout, S. Sundaram, and A. Kalra. 2016. Rapid budding EMS mutants of Synechocystis PCC 6803 producing carbohydrate or lipid enriched biomass. Algal Res. 16: 36–45.
Sankaran, K., C. Naveen, K.K. Vasumathi, M. Premalatha, M. Vijayasekaran, and V.T. Somasundaram. 2015. CDM potential through phycoremediation of industrial effluents. Marine Bioenergy: Trends and Developments. pp. 627-637.
Tanadul, O., J.S. VanderGheynst, D.M. Beckles, A.L.T. Powell and J.M. Labavitch. 2014. The impact of elevated CO2 concentration on the quality of algal starch as a potential biofuel feedstock. Biotechnol. Bioeng. 111(7): 1323-1331.
Vigeolas, H., F. Duby, E. Kaymak, G. Niessen, P. Motte, F. Franck, and C. Remacle. 2012. Isolation and partial characterization of mutants with elevated lipid content in Chlorella sorokiniana and Scenedesmus obliquus. J. Biotechnol. 162: 3–12.
Wang, S., L. Zhang, G. Yang, J. Han, L. Thomsen, and K. Pan. 2016. Breeding 3 elite strains of Nannochloropsis oceanica by nitrosoguanidine mutagenesis and robust screening. Algal Res. 19: 104–108.
Zhang, Y., M. He, S. Zou, C. Fei, Y. Yan, S. Zheng, A.A. Rajper, and C. Wang. 2016. Breeding of high biomass and lipid producing Desmodesmus sp. by ethylmethane sulfonate-induced mutation. Bioresour. Technol. 207: 268–275.
Zhu, B., F. Sun, M. Yang, L. Lu, G. Yang, and K. Pan. 2014. Large-scale biodiesel production using flue gas from coal-fired power plants with Nannochloropsis microalgal biomass in open raceway ponds. Bioresour. Technol. 174: 53–59.
