Effects of Probiotic and Vitamin C on Survival rate and Cortisol Hormone for Broodstock of Thai walking catfish (Clarias macrocephalus)
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Published:
Aug 27, 2018
Keywords:
Probiotic Vitamin C Clarias macrocephalus Growth performance Cortisol Hormone
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Abstract
This study aimed to investigate the effects of probiotic supplement Bacillus subtilis(BS01) and vitamin C derivatives L-ascorbyl-2-monophosphate (AM) in pre-transport Thai walking catfish on survival rate and cortisol hormone for broodstock of Thai walking catfish (Clarias macrocephalus). The experiment was designed using Completely Randomized Design (CRD) by dividing the samples into 6 experimental groups; Group 1 (G1: control), Group 2 (G2: 0.75 g BS01 + 0.25 g Am), Group 3 (G3: 0.50 g BS01 + 0.50 g AM), Group 4 (G4: 0.25 g BS01 + 0.75 g AM), Group 5 (G5:1 g BS 01) and Group 6 (G6: 1 g AM). The results revealed that the initial average weight was 125.00 ± 1.54 g and the density was 20 fish/m2 measured for 90 days of feeding. G6 contributed the highest content of vitamin C (3,371.53 ± 212.67 mg.kg-1) with significant difference among groups observed (P < 0.05). Feed conversion ratio were not different among groups. G6 had the mean of RBC, Hb and HCR at the level of 3.64 ± 0.39x106/mm3, 9.81 ± 3.74 g/dL-1 and 47.13 ± 7.52%, respectively. G6 had the lowest cortisol hormone and serum glucose which were 155.23 ± 52.80 ngml-1 and 112.30 ± 17.50 mmol.L-1, respectively. G4 exhibited the highest Gonadosomatic index (GSI:79.09 ± 5.83% ), fertilized egg (273.00 ± 12.38 egg.g-1) and HR (79.55 ± 1.75%). G5 showed the highest survival rate (87.21 ± 4.07%), which significantly different from G1 (P > 0.05) The water quality indicated by pH value, temperature, DO and TAN of G6 were 5.4 - 7.3, 29 -35 oC, 3.04 -6.24 mg.l-1 and 0.01 - 1.27 mg.l -1, respectively. Therefore, the results of this study showed that dietary supplemented with 1 g.kg-1 Bacillus subtiliscould enhance survival rate and seem to reduce cortisol level of Thai walking catfish.
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Somnuek, T. ., Somnuek, N. ., & Saowakoon, . S. . (2018). Effects of Probiotic and Vitamin C on Survival rate and Cortisol Hormone for Broodstock of Thai walking catfish (Clarias macrocephalus). Khon Kaen Agriculture Journal, 46(4), 643–654. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/250146
Section
บทความวิจัย (research article)
References
ขวัญตา พูลสำราญ ทิพสุคนธ์ พิมพ์พิมล เกรียงศักดิ์เม่งอำพัน และ ชนกันต์ จิตมนัส. 2551. ค่าโลหิตวิทยาของลูกปลาบึก. เชียงใหม่สัตวแพทยสาร 6: 153-163.
คุณาดล ศิลาฤดี. 2550. การศึกษาปริมาณโปรไบโอติก แบคทีเรีย Lactobacillus plantarum CR1T5 ที่เหมาะสมต่อการเจริญเติบโตของปลากดเหลือง. วิทยานิพนธ์ปริญญาวิทยาศาสตรมหาบัณฑิต สาขาวิชาการประมง บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแก่น.
ชาญวิทย์ สุวรรณ์ และ ชนกันต์ จิตมนัส. 2560. การประยุกต์ใช้โพรไบโอติกในการเลี้ยงปลานิล. เชียงใหม่สัตวแพทยสาร. 15: 15-24.
นันทริกา ชันซื่อ และ สมหวัง พิมลบุตร. 2550. การศึกษาเบื้องต้นเกี่ยวกับค่าทางเคมีในเลือดของปลาน้ำจืดในวงศ์ปลาตะเพียนหรือปลาคาร์พ (Family Cyprinidae). Journal of the Thai Veterinary Medical Association under the Royal Patronage. 58: 22-31.
สำนักงานเศรษฐกิจการเกษตร. 2559. ปลาดุก: ผลผลิต รายจังหวัด ปี 2557-2559 (ปี 2558-2559 พยากรณ์ไตรมาส 3 เดือนกันยายน 2559). สำนักงานเศรษฐกิจการเกษตร และคณะทำงานพัฒนาคุณภาพข้อมูลด้านประมง กระทรวงเกษตรและสหกรณ์. สืบค้นจาก https://goo.gl/Tu5PeH สืบค้นเมื่อวันที่ 29 กันยายน 2560.
Aderolu A., F. Zaid, and O. Awobajo. 2017. Dietary effect of Cissus populnea and Securidaca longepedunculata aqueous leave extracts on reproductive. Aceh Journal of Animal Science. 2: 1-11.
Aly, S.M., Y.A-G. Ahmed, A.A-A. Ghareeb, and M.F. Mohamed. 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia niloticus(Oreochromis niloticus) to challenge infection. Fish & Shellfish Immunology. 25: 128-136.
AOAC. 2000. Official methods of analysis (17th ed.). Washington, DC: Association of the Official Analytical Chemists.Arun S., I. Thongam, A. Chanu, I. Mura, and J. Srinivasa. 2017. Virulent Aeromonas veronii Strain BLB-01 Associated with Mass Mortality of Clarias batrachus.
Atrayee D., K. Ghosh, and H. Niladri. 2017. Improvement of Growth and Survival of the Juvenile Walking Catfish, Clarias batrachus(L.) (Siluriformes: Clariidae) Fed on Probiotics Encapsulated and Ascorbic Acid Enriched Chironomid Larvae (Diptera: Chironomidae). Proc Zool.
Boyd, C.E. 1998. Water Quality for Pond Aquaculture. Research and Development Series No. 43. International Center for Aquaculture and Aquatic Environments, Alabama Agricultural Experiment Station, Auburn University, Alabama.
Cabello, F.C. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol. 8: 1137–1144.
Daniel N., A. P. Muralidhar, P. P. Srivastava, K. K. Jain, K. Pani Prasad, J. Manish, and T.Sivaramkrishnan. 2018. Dietary ascorbic acid requirement for growth of striped catfish, Pangasianodon hypophthalmus(Sauvage, 1878) juveniles. Aquaculture Nutrition. 24: 616-624.
Essa, M. A., S. S. EL-Serafy, M. M. El-Ezabi, S. M. Daboor, N. A. Esmael, and S. P. Lall. 2010. Effect of different dietary probiotics on growth, feed utilization and digestive enzymes activities of Nile tilapia, Oreochromis niloticus. Journal of the Arabian Aquaculture Society. 5: 143-162.
Flávia Sampaio D. F., and A. F.Carolina. 2016. An overview of stress physiology of fish transport: Changes in water quality as a function of transport duration. Fish and Fisheries. 17: 1055-1072.
Guo, X., D. Li, W. Lu, X. Piao, and X. Chen. 2006. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilisMA139 in pigs. A. Van. Leeuw. 90: 139–146.
Honggang Z., L. Chao, H. B. Benjamin, R. Zhang, W. Thongda, D. Allen Davis, and E. Peatman. 2015. Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus. Fish & Shellfish Immunology. 46: 624-637.
Iwama, G. K., L. O. B. Afonso, and M. M. Vijayan. 2006. Stress in fishes. In: Evans, D. H. & Claiborne, J. B. The Physiology of fishes. 319-342. Taylor and Francis, 3rd edition. 601 p. USA.
Han, B., W. Long, J. He, Y. Liu, and Y. Si. 2015. Effects of dietary Bacillus licheniformis on growth performance, immunological parameters, intestinal morphology and resistance of juvenile Nile tilapia (Oreochromis niloticus) to challenge infections. Fish. Shellfish. Immunol. 46: 225–231.
Liu, C. H., C. H. Chiu, S. W. Wang, and W.Cheng. 2012. Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of thegrouper, Epinephelus coioides Fish. Shellfish. Immunol. 33: 699–706.
Liu, H., Z. Li, B. Tan, Y. Lao, Z. Duan, W. Sun, and X. Dong. 2014. Isolation of a putative probiotic strain S12 and its effect on growth performance, non-specific immunity and disease resistance of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology. 41: 300-307.
Martinez-Porchas, M., L. R. Martinez-Cordova, and R. Ramos-Enriquez. 2009. Cortisol and glucose: reliable indicators of fish stress?. Pan-American H. of Aquatic Sci. 4:158-178.
Mukherjee, P. 2002. Quality control of herbal drugs. Eastern Publishers (Business Horizons Ltd.) New Delhi.
Na-Nakorn, U., S. Chantsawang, and W. Tarnchalanukit. 1994. Response to mass selection for disease resistance in walking catfish, Clarius macrocephalus. J. Appl. Aqua. 4: 65–73.
Nielsen, S.S. 2010. Vitamin C determination by indophenol method. In Food analysis laboratory manual, vol. 2, ed. Cham: Springer .55-60.
Ogbeche, K.A., Y.O. Ogunbiyi, and F.I.O. Duru. 2002. Effect of Methanol extract of Kigelia africana on Sperm Motility and Fertility in Rats. Nigerian Journal of Health and Biomedical Sciences. 1: 113-116.
Ojha, M.L., N.K. Chadha, V.P. Saini, S. Damroy, Chandraprakash, and P.B. Sawant. 2014. Effect of ethanolic extract of Mucuna pruriens on growth, metabolism and immunity of Labeo rohita (Hamilton, 1822) fingerlings. International Journal of Fauna and Biological Studies. 1: 01-09.
Polakof, S., S. Panserat, J. L. Soengas, and T. W. Moon. 2012. Glucose metabolism in fish: areview. Journal of Comparative Physiology B. 182:1015-1045.
Pottinger, T. G. 1998. Changes in blood cortisol, glucose and lactate in carp retained in anglers’ keepnets. Journal of Fish Biology 53: 728-742.
Robertson L., P. Thomas, C. R. Arnold, and J. M.Trant. 1987. Plasma cortisol and secondary stress responses of red drum to handling, transport, rearing density, and a disease outbreak. The Progressive Fish Culturist. 49: 1-12.
Rodak, B. F., G. A. Fritsma, and K. Doig. 2007. Hematology: Clinical principles and applications. 3rd edition. Saunders Elsevier. Pp. 160-174.
Rotllant, J., P. H. M. Bahm, S. E. Wendelaar-Bonga, J. Perez-Sanchez, and L. Tort. 2000. A drop in ambient temperature results in a transient reduction of interrenal ACTH responsiveness in the gilthead sea bream (Sparus aurata L.). Fish Physiol. Biochem. 23: 265-273.
Smith, P. 2008. Antimicrobial resistance in aquaculture. Rev. Sci.Technol. 27: 243–264.
Tongpim, S., R. Meidong, P. Poudel, S. Yoshino, and Y. Okugawa. 2014. Isolation of thermophilic L-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition. J. Biosci. Bioeng. 117: 318–324.
คุณาดล ศิลาฤดี. 2550. การศึกษาปริมาณโปรไบโอติก แบคทีเรีย Lactobacillus plantarum CR1T5 ที่เหมาะสมต่อการเจริญเติบโตของปลากดเหลือง. วิทยานิพนธ์ปริญญาวิทยาศาสตรมหาบัณฑิต สาขาวิชาการประมง บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแก่น.
ชาญวิทย์ สุวรรณ์ และ ชนกันต์ จิตมนัส. 2560. การประยุกต์ใช้โพรไบโอติกในการเลี้ยงปลานิล. เชียงใหม่สัตวแพทยสาร. 15: 15-24.
นันทริกา ชันซื่อ และ สมหวัง พิมลบุตร. 2550. การศึกษาเบื้องต้นเกี่ยวกับค่าทางเคมีในเลือดของปลาน้ำจืดในวงศ์ปลาตะเพียนหรือปลาคาร์พ (Family Cyprinidae). Journal of the Thai Veterinary Medical Association under the Royal Patronage. 58: 22-31.
สำนักงานเศรษฐกิจการเกษตร. 2559. ปลาดุก: ผลผลิต รายจังหวัด ปี 2557-2559 (ปี 2558-2559 พยากรณ์ไตรมาส 3 เดือนกันยายน 2559). สำนักงานเศรษฐกิจการเกษตร และคณะทำงานพัฒนาคุณภาพข้อมูลด้านประมง กระทรวงเกษตรและสหกรณ์. สืบค้นจาก https://goo.gl/Tu5PeH สืบค้นเมื่อวันที่ 29 กันยายน 2560.
Aderolu A., F. Zaid, and O. Awobajo. 2017. Dietary effect of Cissus populnea and Securidaca longepedunculata aqueous leave extracts on reproductive. Aceh Journal of Animal Science. 2: 1-11.
Aly, S.M., Y.A-G. Ahmed, A.A-A. Ghareeb, and M.F. Mohamed. 2008. Studies on Bacillus subtilis and Lactobacillus acidophilus, as potential probiotics, on the immune response and resistance of Tilapia niloticus(Oreochromis niloticus) to challenge infection. Fish & Shellfish Immunology. 25: 128-136.
AOAC. 2000. Official methods of analysis (17th ed.). Washington, DC: Association of the Official Analytical Chemists.Arun S., I. Thongam, A. Chanu, I. Mura, and J. Srinivasa. 2017. Virulent Aeromonas veronii Strain BLB-01 Associated with Mass Mortality of Clarias batrachus.
Atrayee D., K. Ghosh, and H. Niladri. 2017. Improvement of Growth and Survival of the Juvenile Walking Catfish, Clarias batrachus(L.) (Siluriformes: Clariidae) Fed on Probiotics Encapsulated and Ascorbic Acid Enriched Chironomid Larvae (Diptera: Chironomidae). Proc Zool.
Boyd, C.E. 1998. Water Quality for Pond Aquaculture. Research and Development Series No. 43. International Center for Aquaculture and Aquatic Environments, Alabama Agricultural Experiment Station, Auburn University, Alabama.
Cabello, F.C. 2006. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ. Microbiol. 8: 1137–1144.
Daniel N., A. P. Muralidhar, P. P. Srivastava, K. K. Jain, K. Pani Prasad, J. Manish, and T.Sivaramkrishnan. 2018. Dietary ascorbic acid requirement for growth of striped catfish, Pangasianodon hypophthalmus(Sauvage, 1878) juveniles. Aquaculture Nutrition. 24: 616-624.
Essa, M. A., S. S. EL-Serafy, M. M. El-Ezabi, S. M. Daboor, N. A. Esmael, and S. P. Lall. 2010. Effect of different dietary probiotics on growth, feed utilization and digestive enzymes activities of Nile tilapia, Oreochromis niloticus. Journal of the Arabian Aquaculture Society. 5: 143-162.
Flávia Sampaio D. F., and A. F.Carolina. 2016. An overview of stress physiology of fish transport: Changes in water quality as a function of transport duration. Fish and Fisheries. 17: 1055-1072.
Guo, X., D. Li, W. Lu, X. Piao, and X. Chen. 2006. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilisMA139 in pigs. A. Van. Leeuw. 90: 139–146.
Honggang Z., L. Chao, H. B. Benjamin, R. Zhang, W. Thongda, D. Allen Davis, and E. Peatman. 2015. Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus. Fish & Shellfish Immunology. 46: 624-637.
Iwama, G. K., L. O. B. Afonso, and M. M. Vijayan. 2006. Stress in fishes. In: Evans, D. H. & Claiborne, J. B. The Physiology of fishes. 319-342. Taylor and Francis, 3rd edition. 601 p. USA.
Han, B., W. Long, J. He, Y. Liu, and Y. Si. 2015. Effects of dietary Bacillus licheniformis on growth performance, immunological parameters, intestinal morphology and resistance of juvenile Nile tilapia (Oreochromis niloticus) to challenge infections. Fish. Shellfish. Immunol. 46: 225–231.
Liu, C. H., C. H. Chiu, S. W. Wang, and W.Cheng. 2012. Dietary administration of the probiotic, Bacillus subtilis E20, enhances the growth, innate immune responses, and disease resistance of thegrouper, Epinephelus coioides Fish. Shellfish. Immunol. 33: 699–706.
Liu, H., Z. Li, B. Tan, Y. Lao, Z. Duan, W. Sun, and X. Dong. 2014. Isolation of a putative probiotic strain S12 and its effect on growth performance, non-specific immunity and disease resistance of white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology. 41: 300-307.
Martinez-Porchas, M., L. R. Martinez-Cordova, and R. Ramos-Enriquez. 2009. Cortisol and glucose: reliable indicators of fish stress?. Pan-American H. of Aquatic Sci. 4:158-178.
Mukherjee, P. 2002. Quality control of herbal drugs. Eastern Publishers (Business Horizons Ltd.) New Delhi.
Na-Nakorn, U., S. Chantsawang, and W. Tarnchalanukit. 1994. Response to mass selection for disease resistance in walking catfish, Clarius macrocephalus. J. Appl. Aqua. 4: 65–73.
Nielsen, S.S. 2010. Vitamin C determination by indophenol method. In Food analysis laboratory manual, vol. 2, ed. Cham: Springer .55-60.
Ogbeche, K.A., Y.O. Ogunbiyi, and F.I.O. Duru. 2002. Effect of Methanol extract of Kigelia africana on Sperm Motility and Fertility in Rats. Nigerian Journal of Health and Biomedical Sciences. 1: 113-116.
Ojha, M.L., N.K. Chadha, V.P. Saini, S. Damroy, Chandraprakash, and P.B. Sawant. 2014. Effect of ethanolic extract of Mucuna pruriens on growth, metabolism and immunity of Labeo rohita (Hamilton, 1822) fingerlings. International Journal of Fauna and Biological Studies. 1: 01-09.
Polakof, S., S. Panserat, J. L. Soengas, and T. W. Moon. 2012. Glucose metabolism in fish: areview. Journal of Comparative Physiology B. 182:1015-1045.
Pottinger, T. G. 1998. Changes in blood cortisol, glucose and lactate in carp retained in anglers’ keepnets. Journal of Fish Biology 53: 728-742.
Robertson L., P. Thomas, C. R. Arnold, and J. M.Trant. 1987. Plasma cortisol and secondary stress responses of red drum to handling, transport, rearing density, and a disease outbreak. The Progressive Fish Culturist. 49: 1-12.
Rodak, B. F., G. A. Fritsma, and K. Doig. 2007. Hematology: Clinical principles and applications. 3rd edition. Saunders Elsevier. Pp. 160-174.
Rotllant, J., P. H. M. Bahm, S. E. Wendelaar-Bonga, J. Perez-Sanchez, and L. Tort. 2000. A drop in ambient temperature results in a transient reduction of interrenal ACTH responsiveness in the gilthead sea bream (Sparus aurata L.). Fish Physiol. Biochem. 23: 265-273.
Smith, P. 2008. Antimicrobial resistance in aquaculture. Rev. Sci.Technol. 27: 243–264.
Tongpim, S., R. Meidong, P. Poudel, S. Yoshino, and Y. Okugawa. 2014. Isolation of thermophilic L-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition. J. Biosci. Bioeng. 117: 318–324.
