Evaluation of cherry tomato resistant to Tomato yellow leaf curl Thailand virus (TYLCTHV) and validated markers associated with Ty-2 and Ty-3 resistant genes
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Abstract
Tomato yellow leaf curl disease caused by Begomovirus of the family Geminiviridaeis serious disease of tomato (Solanum lycopersicum L.) production particularly in sub-tropic and tropic regions like Thailand. Host resistance is sustainable disease control. The aims of this study were selected cherry tomato varieties gene resistance to Tomato yellow leaf cure virus (Thai isolate), high fruit yield and high quality (brix and vitamin C). Nine cherry tomatoes, 1 susceptible check and 2 resistant checks were used for this study. The Randomized completed block design (RCBD) with three replications and 10 plants of each were designed. The plants were inoculated with TYLCTHV isolate by grafting method for phenotyping and Two SCAR markers were used for genotyping. The cherry tomato variety, GT-1-2-7, was resistant to the disease and it contained Ty-3/ty-3 gene. Additionally, the resistant check varieties also showed Ty-2/Ty-2 and Ty-3/Ty-3genes. The highest yield was found in CHRY, Cindy sweet and Maneetabtim (839.10, 742.00 and 563.30 g/plant, respectively). Maneetabtim, Red lady and Yellow Sweet gave the highest total soluble solids (11, 10 and 9.74, respectively). The highest vitamin C concentrated was found highest in GT-1-2-7 and VF-134 varieties with value (3.10 and 2.46 mg/100 g fresh weight, respectively). Therefore, six tomato varieties including GT1-2-7, CHRY, Red lady, Yellow sweet, Cindy sweet and Maneetabtim variety should be used as high potential varieties for improving new variety with high yield, good quality and resistant to TYLCVTH disease.
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