Assessment of Genetic Relationship in 29 Thai pumpkin cultivars using AFLP markers
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Abstract
There are several commercial pumpkin cultivars that were selected for similar characteristics such as fruit shape, fresh color, and skin type, and skin color. The hypothesis of this experiment was pumpkin cultivars including commercial cultivar and landrace cultivar in Thailand that are similar of genetic base. The objective of the study is to evaluate genetic diversity of 29 pumpkin cultivars including 25 commercial cultivars (five open pollinated cultivars and 20 F1 hybrid cultivars) and four landrace cultivars using Amplified Fragment Length Polymorphisms (AFLP) markers. From 28 AFLP primers, seven primers were able to amplify 168 polymorphic bands. All cultivars were clustered using UPGMA (Unweighted pair group method with arithmetic average) method based on dice similarity coefficient. Dice similarity coefficients are between 0.21 and 0.96 with the average 0.81. Twenty nine pumpkin cultivars were grouped into 2 groups which group1 included 23 cultivars that were both commercial and landrace cultivars. Group 2included two cultivars and four cultivars were out group. When Neighbor-joining clustering method based on standard genetic distance was used, 29 pumpkin cultivars were grouped into3 groups and six pumpkin cultivars were out group. From two clustering methods, Thai commercial pumpkin cultivars currently was similarity genetic base and five pumpkin cultivars that are Tung, K-Tone, KPS-1, CM-1, and EP selected for parent line in pumpkin breeding program.
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ปณาลี ภู่วรกุลชัย, สราวุฒิ เกตุแก้ว, เขมวรรณ ศรีตงกิม, บุบผา คงสมัย และอัญมณี อาวุชานนท์. 2555. การศึกษาความสัมพันธ์ทางพันธุกรรมด้วยเครื่องหมายดีเอ็นเอ SRAP และลักษณะคุณภาพผลของฟักทองพันธุ์การค้าของไทยบางพันธุ์. การประชุมวิชาการแห่งชาติ มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกำแพงแสน ครั้งที่ 9. 1100-1106.
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