Patterns of cGH, IGF-1, ApoB2, ApoVLDL-II and FASN; and body weight and cholesterol level in plasma of Thai native chicken crossbreds

Abstract

The objective of this study was to identify the patterns of cGH, IGF-1, ApoB2, ApoVLDL-II and FASN that may be associated with body weight and blood cholesterol level of 25, 50 and 75% crossbred Thai native chickens. A total of 120 samples were collected and performed on using PCR-RFLP analysis. The results found that there were three patterns of cGH, IGF-1, ApoB2 and FASN; whereas two patterns were observed in ApoVLDL-II. An association analysis showed a significant difference between variations of FASN and body weight at 2 weeks. AA genotype of FASN had higher body weight at 2 weeks than that of genotypes AG and GG. No association between the variation of genes and cholesterol level was found in 25%, 50% and 75% crossbreds. In addition, the results found association between gender and body weight and cholesterol level in plasma at 10 weeks. Males were heavier than females as well as had higher cholesterol level in plasma. In 75% Thai native chicken crossbreds, the body weight and cholesterol level at 10 weeks were lower than those of 25% and 50% Thai native chicken crossbreds. Although no association of body weight and cholesterol level was investigated, the variation of FASN detected in this study might underpin the development of genetic markers for improved body weight at 2 weeks in Thai native chicken crossbreds.