Microsatellite markers selection for detected of Holstein blood level in crossbred dairy cows

Abstract

The objective of this study is to fnd the microsatellite markers which can detect the different blood levels of Holstein-Friesians in crossbred dairy cattle. Three groups of 20 crossbred dairy cattle with blood levels of 50%, 75% and 100%, respectively, were studied. The correlation between 10 microsatellite markers, i.e. BM1824, BM2113, BM4305, BMS2684, ETH3, ETH152, ETH225, ILST096, TGLA122 and TGLA126 was examined using Binary Logistic Regression Analysis. It was found that the marker TGLA122 has the possibility of identifying 50% Holstein-Friesian crossbred dairy cattle, with the probabilities of allele B and genotype BB at 91.83% and 96%, respectively. In 75% and 100% Holstein-Friesian crossbred dairy cattle, none of the 10 microsatellite markers were found to be capable of identifying these blood levels. However, even though the results of this study showed that only one microsatellite marker can be used for the detection of blood levels of Holstein-Friesians, these can form the basis and be used to fnd the microsatellite marker groups which can detect the blood levels of Holstein-Friesians in crossbred dairy cattle.