Bioplastic production from bananas by soil isolating bacteria
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Published:
May 18, 2021
Keywords:
bioplastic polyhydroxyalkanotes banana Klebsiella pneumoniae
Main Article Content
Abstract
The research aims to study the bioplastic of polyhydroxyalkanoates (PHAs) production by bacteria isolated from soil samples of Chachoengsao and Nakhon Nayok provinces using 3 kind of ripe banana; Lady Finger, Cavendish and Pisang Awak as carbon sources. Among only 16 isolates can produced PHAs. However, the maximum PHAs content (30.10±0.25%) was obtained from the isolate strain N22-3. The cell morphology was investigated by the Gram staining method using the light microscope. It was found that the N22-3 isolate was a Gram-negative. Then it was identified by 16S rRNA analysis and showed that the PHAs-producing bacterium had maximum sequence 99.85% similarity with Klebsiella pneumoniae. The PHAs film was characterized in its structure. The result showed that it was similar to the standard biopolymer of polyhydroxybutyrate (PHB).
Article Details
How to Cite
Singhaboot, P. ., Apiwun, P. ., Panthong, P. ., & Kroeksakul, P. . (2021). Bioplastic production from bananas by soil isolating bacteria. Khon Kaen Agriculture Journal, 49(3), 668–678. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/250847
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บทความวิจัย (research article)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
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Bengtsson, S., A. Werker, M. Christensson, and T. Welander. 2008. Production of polyhydroxy lalkanoates by activated sludge treating a paper mill wastewater. Bioresource Technology. 99: 509-511.
Bernard, M. 2014. Industrial potential of polyhydroxyalkanoate bioplastic: a brief review. University of Saskatchewan Undergraduate Research Journal. 1: 1-14.
Burdon, K. L. 1946. Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. Journal of Bacteriology. 52: 665-678.
Cavalheiro, J. M. B. T., M. C. M. D. Almeida, C. Grandfils, and M. M. R. Fonseca. 2009. Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochemistry. 44: 509-515.
Chen, G. Q. 2009. A microbial polyhydroxyl alkanoates (PHA) based bio-and materials industry. Chemical Society Reviews. 38: 2434-2466.
Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28: 350-356.
El-Sayed, A. A., H. M. Abdelhady, A. M. Hafez, and T. A. Khodair. 2009. Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. Journal of Applied Sciences Research. 5: 556-564.
Getachew, A., and F. Woldesenbet. 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes. 9: 509.
Grothe, E., M. Moo-Young, and Y. Chisti. 1999. Fermentation optimization for the production of poly (ß-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microbial Technology. 25:132-141.
Gouda, M. K., A. E. Swellam, and S. H. Omar. 2001. Production of PHB by Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological Research. 156: 201-207.
Mezzolla, V., O. F. D’Urso, and P. Poltronieri. 2017. Optimization of polyhydroxyalkanoate production by recombinant E. coli supplemented with different plant by-products. BioTechnology: An Indian Journal. 13: 138-155.
Janes B., J. Hollar, and D. Dennis. 1990. Molacular characterization of the poly-β-hydoxybutyrate biosynthetic pathway of Alcaligenes eutrophus H16. In: Novel biodegradable microbial polymer. (Eds: Dawes, E.A.). The Netherlands. Kluwer Academic Publisher. Dordrecht.
Kunasundari, B., and K. Sudesh. 2011. Isolation and recovery of microbial polyhydroxyalkanoates. eXPRESS Polymer Letters. 5: 620-634.
Nandini, P., C. Amruta, P. Bhavesh, R. Pragya, V. Priti, and P. Mital. 2011. Screening of PHB (polyhydroxyalkanoates) producing bacteria from diverse sources. Microbial Biotechnology. 36: 216-274.
Phathipchotikun, R., P. Piwpan, A. Jaturapiree, and P. Jaturapiree. 2014. Polyhydroxybutyrate (PHB) production by Alcaligenes eutrophus NCIMB 11599 from low-cost substrate as carbon source. KKU Research Journal. 19(Supplement Issue): 53-59.
Santhanam, A., and S. Sasidharan. 2010. Microbial production of polyhydroxyalkanotes (PHA) from Alcaligenes spp. and Pseudomonas oleovorans using different carbon sources. African Journal of Biotechnology. 9: 3144-3150.
Singhaboot, P., and P. Kaewkannetra. 2015. A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice. Annals of Microbiology. 65: 2081-2089.
Suwannasing, W., S. Moonamart, and P. Kaewkannetra. 2011. Yields of polyhydroxyalkanoates (PHAs) during batch fermentation of sugar cane juice by Alcaligenes latus and Alcaligenes eutrophus. Life Science Journal. 5: 960-966
Suwannasing, W., T. Imai, and P. Kaewkannetra. 2015. Cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials. Bioresource Technology. 194: 67-74.
Tanamool, V., T. Imai, P. Danvirutai, and P. Kaewkannetra. 2013. Biopolymer generation from sweet sorghum juice: screening, isolation, identification, and fermentative polyhydroxyalkanoate production by Bacillus aryabhattai. Turkish Journal of Biology. 37: 259-264.
Tufail, S., S. Munir, and N. Jamil. 2017. Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons. Brazilian Journal of Microbiology. 48: 629-636.
Vega, E. Z., B. A. Glatz, and E. G. Hammond. 1988. Optimization of banana juice fermentation for the production of microbial oil. Applied and Environmental Microbiology. 54: 748-752.
Abou-Zeid, D. M. 2001. Anaerobic biodegradation of natural and synthetic polyesters. Ph.D. Dissertations in Science, Technical University Carolo-Wilhelmina at Brunswick, Germany.
Bengtsson, S., A. Werker, M. Christensson, and T. Welander. 2008. Production of polyhydroxy lalkanoates by activated sludge treating a paper mill wastewater. Bioresource Technology. 99: 509-511.
Bernard, M. 2014. Industrial potential of polyhydroxyalkanoate bioplastic: a brief review. University of Saskatchewan Undergraduate Research Journal. 1: 1-14.
Burdon, K. L. 1946. Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. Journal of Bacteriology. 52: 665-678.
Cavalheiro, J. M. B. T., M. C. M. D. Almeida, C. Grandfils, and M. M. R. Fonseca. 2009. Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochemistry. 44: 509-515.
Chen, G. Q. 2009. A microbial polyhydroxyl alkanoates (PHA) based bio-and materials industry. Chemical Society Reviews. 38: 2434-2466.
Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28: 350-356.
El-Sayed, A. A., H. M. Abdelhady, A. M. Hafez, and T. A. Khodair. 2009. Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. Journal of Applied Sciences Research. 5: 556-564.
Getachew, A., and F. Woldesenbet. 2016. Production of biodegradable plastic by polyhydroxybutyrate (PHB) accumulating bacteria using low cost agricultural waste material. BMC Research Notes. 9: 509.
Grothe, E., M. Moo-Young, and Y. Chisti. 1999. Fermentation optimization for the production of poly (ß-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microbial Technology. 25:132-141.
Gouda, M. K., A. E. Swellam, and S. H. Omar. 2001. Production of PHB by Bacillus megaterium strain using sugarcane molasses and corn steep liquor as sole carbon and nitrogen sources. Microbiological Research. 156: 201-207.
Mezzolla, V., O. F. D’Urso, and P. Poltronieri. 2017. Optimization of polyhydroxyalkanoate production by recombinant E. coli supplemented with different plant by-products. BioTechnology: An Indian Journal. 13: 138-155.
Janes B., J. Hollar, and D. Dennis. 1990. Molacular characterization of the poly-β-hydoxybutyrate biosynthetic pathway of Alcaligenes eutrophus H16. In: Novel biodegradable microbial polymer. (Eds: Dawes, E.A.). The Netherlands. Kluwer Academic Publisher. Dordrecht.
Kunasundari, B., and K. Sudesh. 2011. Isolation and recovery of microbial polyhydroxyalkanoates. eXPRESS Polymer Letters. 5: 620-634.
Nandini, P., C. Amruta, P. Bhavesh, R. Pragya, V. Priti, and P. Mital. 2011. Screening of PHB (polyhydroxyalkanoates) producing bacteria from diverse sources. Microbial Biotechnology. 36: 216-274.
Phathipchotikun, R., P. Piwpan, A. Jaturapiree, and P. Jaturapiree. 2014. Polyhydroxybutyrate (PHB) production by Alcaligenes eutrophus NCIMB 11599 from low-cost substrate as carbon source. KKU Research Journal. 19(Supplement Issue): 53-59.
Santhanam, A., and S. Sasidharan. 2010. Microbial production of polyhydroxyalkanotes (PHA) from Alcaligenes spp. and Pseudomonas oleovorans using different carbon sources. African Journal of Biotechnology. 9: 3144-3150.
Singhaboot, P., and P. Kaewkannetra. 2015. A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice. Annals of Microbiology. 65: 2081-2089.
Suwannasing, W., S. Moonamart, and P. Kaewkannetra. 2011. Yields of polyhydroxyalkanoates (PHAs) during batch fermentation of sugar cane juice by Alcaligenes latus and Alcaligenes eutrophus. Life Science Journal. 5: 960-966
Suwannasing, W., T. Imai, and P. Kaewkannetra. 2015. Cost-effective defined medium for the production of polyhydroxyalkanoates using agricultural raw materials. Bioresource Technology. 194: 67-74.
Tanamool, V., T. Imai, P. Danvirutai, and P. Kaewkannetra. 2013. Biopolymer generation from sweet sorghum juice: screening, isolation, identification, and fermentative polyhydroxyalkanoate production by Bacillus aryabhattai. Turkish Journal of Biology. 37: 259-264.
Tufail, S., S. Munir, and N. Jamil. 2017. Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons. Brazilian Journal of Microbiology. 48: 629-636.
Vega, E. Z., B. A. Glatz, and E. G. Hammond. 1988. Optimization of banana juice fermentation for the production of microbial oil. Applied and Environmental Microbiology. 54: 748-752.
