Detection of quantitative trait loci controlling fiber content and pod length in yardlong bean by SNP molecular marker
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Abstract
Yardlong bean [Vigna unguiculata subsp. sesquipedalis (L.) Verdc.] is one of an economically important vegetable legume crop in the Thailand. Ngu bean is a type of yardlong beans in which the pod texture is crispy, curly, wrinkled, rougher, thicker and shorter than yardlong beans. The different of texture between yardlong bean and Ngu bean is expected due to the amount of fiber in bean pod: cellulose, hemicellulose and lignin. The objective of this study is to identify the quantitative trait loci (QTLs) controlling pod length and the amount of cellulose, hemicellulose and lignin of yardlong bean. An F2 population of cross between Ngu bean and yardlong bean cultivar “Raya” was used to construct genetic map of Single Nucleotide Polymorphism (SNP) markers and evaluate for pod length and pod fiber content. The pod length of F2 population ranged from 23.7-75.5 cm. The fiber content of cellulose, hemicellulose and lignin varied from 18.03-25.16, 5.87-12.07 and 1.56-8.77%, respectively. A genetic map containing 441 SNPs was constructed and used to identify six quantitative trait loci (QTLs) for these traits. Four QTLs responsible for pod length were found one each on LG4 and LG7.2 and 2 QTLs on LG8. QTL analysis detected two QTLs related for cellulose content on LG8 and LG10. Moreover, QTL of cellulose content on LG8 was similar to LG1 and QTL of pod length on LG4 and LG8 were similar to LG7 and LG3 which were identified by previous work
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