Digestive Enzyme Activity in Adult of Striped Catfish (Pangasianodon hypophthalmus)
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Abstract
Study of digestive enzyme activities, protease, lipase, amylase and cellulase in liver, stomach, anterior and posterior intestine of striped catfish with average mean weight of 210±8.5 g/fish. The results of estimated enzyme activities in liver, stomach, anterior and posterior intestine were found that protease to be highest activity at pH 12, 12, 10 and 9 with level 0.295±0.02, 0.201±0.00, 0.200±0.01 and 0.033±0.01 Unit/mg Protein respectively. Lipase was the highest activity at pH 12, 12, 12 and 4 respectively. In a stomach and posterior intestine were no significantly at 1175.51±50.24 and 1249.20±55.30. Amylase showed the highest activity at pH 10, 4-5, 7-8 and 4-5 by liver has performed the highest activity level (P< 0.05) at 0.156±0.10 Unit/mg Protein, followed by stomach, anterior and posterior intestine which performed the activity level at 0.153±0.05, 0.092±0.02 and 0.047±0.00 Unit/mg Protein respectively. Cellulase was found that the highest activity at pH 12, 5, 5 and 7 by liver has performed the highest activity level at 0.026±0.01 Unit/mg Protein. The highest lipase activity was observed in striped catfish, followed by protease, amylase and cellulase. The activity of digestive enzymes of striped catfish might interaction with nutrients derivation. This basic information can be used for the selection of raw materials for striped catfish feed formulation.
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การุณ ทองประจุแก้ว. 2554. การพัฒนาสูตรอาหารโดยใช้เทคโนโลยีของเอนไซม์ย่อยอาหารเพื่อการเจริญเติบโตอย่างมีคุณภาพของปลากัด (Betta splendens Regan, 1910). วิทยานิพนธ์ปริญญาดุษฎีบัณฑิต มหาวิทยาลัยเกษตรศาสตร์, กรุงเทพมหานคร.
จันทกานต์ นุชสุข, อรุณี อิงคากุล, อุทัยวรรณ โกวิทวที และ อรพินท์ จินตสถาพร. 2549. คุณลักษณะของเอนไซม์ย่อยอาหารในปลาสวายหนู Heligophagus leptrorhynchus Ng&Kottelat, 2000. ใน: การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ครั้งที่ 45 (สาขาประมง). มหาวิทยาลัยเกษตรศาสตร์, กรุงเทพมหานคร.
ยุทธนา เอียดน้อย, อรุณี อิงคากุล, อุทัยวรรณ โกวิทวที และ สุริยัน ธัญกิจจานุกิจ. 2550. คุณลักษณะและประสิทธิภาพของเอนไซม์ย่อยอาหารในกุ้งกุลาดำ Penaeus monodon, กุ้งขาว Penaeus vannamei, และกุ้งก้ามกราม Macrobrachium rosenbergii. วารสารวิจัยเทคโนโลยีการประมง 1: 248-260.
รุ่งกานต์ กล้าหาญ, นนทวิทย์ อารีย์ชน, เรืองวิชญ์ ยุ้นพันธ์ และ อรุณี อิงคากุล. 2551. กิจกรรมของเอนไซม์ย่อยอาหารในปลานิล (Oreochromis niloticus, L.) ที่ขนาดต่างๆ. วารสารวิจัยเทคโนโลยีการประมง 2: 33-43.
Areekijseree, M., A. Engkagulb, U. Kovitvadhic, A. Thongpand, M. Mingmuangd, P. Pakkonge, and K. Rungruangsak-Torrissenf. 2004. Temperature and pH characteristics of amylase and proteinase of adult freshwater pearl mussel, Hyriopsis (Hyriopsis) bialatus Simpson 1900. Aquaculture. 234: 575–587.
Alarcon, F.J., M. Díaz-López, and F.J. Moyano. 1997. Studies on Digestive Enzymes in Fish: Characterization and Practical Applicatio. Cah. Opt. Mediterr. 22: 113-121.
Bernfeld, P. 1955. Enzymes of carbohydrate metabolism. In: Colowick, S.P., Kaplan, N.O. (Eds.) Method in Enzymology, vol. 1. Academic Press, New York.
Bezerra, R. S., E. J. F. Lins, R. B. Alencar, P. M. G. Paiva, M. E. C. Chaves, L. C. B. B. Coelho, and L. B. Carvalho Jr. 2005. Alkaline proteinase from intestine of Nile tilapia (Oreochromis niloticus). Process Biochem. 40: 1829-1834.
Chakrabarti, I., A. Gani Md, K. K. R. Chaki Sur, and K. K. Misra. 1995. Digestive enzymes in 11 freshwater teleost fish species in relation to food habit and niche segregation. Camp. Biochem. Physiol. 1: 167-177.
Chakrabarti, R., R. M. Rathore, P. Mittal, and Kumar, S. 2006. Functional changes in digestive enzymes and characterization of proteases of silver carp (♂) and bighead carp (♀) hybrid, during early ontogeny. Aquaculture. 253: 694–702.
Chiu, Y.N., and L. V. Benitez. 1981. Studies on the carbohydrases in the digestive tract of the milkfish Chanos chanos. Mar. Biol. 61: 247–254.
Das, K.M., and S. D. Tripathi. 1991. Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.). Aquaculture. 92: 21– 32.
De Silva S.S. and T. A. Anderson. 1995. Fish Nutrition in Aquaculture. Chapman & Hall Aquaculture Series, London.
El-Beltagy, A. E., T. A. El-Adawy, E. H. Rahma, and A. A. El-Bedawey. 2004. Purification and characterization of an acidic protease from the viscera of bolti fish (Tilapia nilotica). Food Chem. 86: 33–39.
Fagbenro, O.A. 1990. Food composition and digestive enzymes in the gut of pond-cultured Clarias isheriensis (Sydenham 1980), (Siluriformes Clariidae). J. Appl. Ichthyol. 6: 91–98.
Furne, M.,M. C. Hidalgo, A. Lo´pez, M. Garcı´a-Gallego, A. E. Morales, A. Domezain, J. S. Domezaine, and A. Sanz. 2005. Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comparative study. Aquaculture. 250: 391– 398.
Gaxiola, G., G. Cuzon, T. García, G. Taboada, R. Brito, M. E. I. Chima, A. Paredes, L. Soto, C. Rosas, and A. V. Wormhoudt. 2005. Factorial effects of salinity, dietary carbohydrate and moult cycle on digestive carbohydrases and hexokinases in Litopenaeus vannamei (Boone, 1931). Comp. Biochem. Physiol. A. 140: 29-3.
Halver, J. E. and R. W. Hardy. 2002. Fish Nutrition. Academic Press, United States of America.
De Silva, S.S., and T. A. Anderson. 1995. Fish Nutrition in Aquaculture. Chapman & Hall, UK.
Hidalgo, M.C., E. Urea, and A. Sanz. 1999. Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture. 170: 267– 283.
Klahan, R., N. Areechon, R. Yoonpundh, and A. Engkagul. 2009. Characterization and Activity of Digestive Enzymes in Different Sizes of Nile Tilapia (Oreochromis niloticus L.). Kasetsart. J. Nat. Sci. 43.
Laura, E., Celis-Guerrero, L. Fernando, Garcia-Carreno, and M. Angeles Navarrete del Toro. 2004. Characterization of Proteases in the Digestive System of Spiny Lobster (Panulirus interruptus). Mar. Biotechnol. 6: 262–269.
Lindsay, G. J . H., and J. E. Harris. 1980. Carboxymethylcellulase activity in the digestive tract of fish. J. Fish Biol. 16: 219–233.
Lowry, H.O., J. N. Rosebrough, A. L. Farr, and R. J. I. Randal. 1951. Protein measurements with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
Markweg, H., M. S. Lang, and F. Wagner. 1995. Dodecanoic acid inhibition of lipase from Acinetobacter sp. OPA 55. Enz. Microb. Tech. 17: 512 – 516.
Miller, G. L. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal. Chem. 31: 426–428.
Moreau, Y., V. Desseaux, R. Koukiekolo, G. M. Mouren, and M. Santimone. 2001. Starch digestion in tropical fishes: structural studies and inhibition kinetics of α - amylase from two tilapias Oreochromis niloticus and Sarotherodon melanotheron. Comp. Biochem. Physiol. B. 128: 543 – 552.
Natalia, Y., R. Hashim, A. Ali, and A. Chong. 2004. Characterization of digestive enzymes in a canivoous ornamental fish, the Asian bony tongue Scleropages formosus (Osteoglossidae). Aquaculture. 233:305-320.
Pan, L., G. Xiao, H. Zhang, and Z. Luan. 2005. Effects of different dietary Protein content on growth and protease activity of Eriocheir sinensis larvae. Aquaculture. 246: 313-319.
Paripatananont, T. 2002. Snakehead and Pangasius catfish. Nutrient Requirements and Feeding of Finfish for Aquaculture. CAB International.
Castro P, F., C. V. Augusto, Jr. Freitas, M. S. Werlayne, M. S. Helane, L. B. Costa, Jr. Carvalho, and Ranilson S. Bezerra. 2012. Comparative Study of Amylases from the Midgut Gland of Three Species of Penaeid Shrimp. J. Crustacen Biol. 32: 607-613.
Sheng, L. J., L. J. Lin, and W. T. Ting. 2006. Ontogeny of protease, amylase and lipase in the alimentary tract of hybrid Juvenile tilapia (Oreochromis niloticus X Oreochromisaureus). Fish Physiol. Biochem. 32: 295–303.