Detection of fungal pathogen causing durian dieback disease
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Abstract
Misconception of a fungal pathogen causing durian die back was Phytophthora palmivora, durian growers have been conducted inaccurate fungicides to eliminate the target fungus likely started a long time ago. Die back still be scattered in the durian orchards causing yellow leaves and will fall-off, then leaving absolutely. The aim of this research was to investigate the certain causal agent of durian die back disease for outbreak prevention. Dieback symptom of durian branches, brown lesion of stem and soil samples were collected from the orchards in Chumphon, Chanthaburi, Trat provinces. Fungal pathogens of 28 isolates were identified and isolated by using tissue transplanting technique with potato dextrose agar (PDA) and BNCRA selective medium based on morphological characteristics including molecular markers inspection. The ITS region (ITS1-5.8s-ITS2) and TEF1-alpha contained sequences approximately 800 and 1,000 bp, respectively. Nucleotide sequences were analyzed and identified as Fusarium solani. Also, pathogenicity test was investigated on 6-month-old durian seedlings by artificial inoculation with wounded and non-wounded inoculations. The results showed that disease symptom occurring after 3 days of both inoculations with light brown lesion outside and light brown to dark brown inside of inoculated durian branches.
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References
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