Effects of butyrate salts on growth performances, intestinal morphological structure and caecal microbial population of broilers
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Published:
Aug 26, 2020
Keywords:
butyrate salts growth performances intestinal morphological structure caecal microbial population broilers
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Abstract
This study was designed to examine the effects of butyrate salts on growth performances, intestinal morphological structure and caecal microbial population of broilers. In total 1,248 one-day old chicks were randomly allocated into 4 groups with 12 replicates, which consisted of 26 birds with equal male and female, and were randomly fed the experimental diets as following: a control diet based on corn and soybean meal, control diets supplemented with 8 mg/kg flavophopholipol (as antibiotic growth promoter: AGP), 1,000 mg/kg sodium butyrate and 300 mg/kg calcium butyrate as group 2 to 4, respectively. The supplementation of AGP significantly improved the feed conversion ratio of broiler in period 1-10 day of ages (DOA) compared with control group (P<0.01), whereas butyrate salts either sodium or calcium salts provided the non-significant effect on growth performances. However, the growth performances of broilers fed butyrate salts in period 21-35 DOA has a tendency to be better than control and AGP group (P=0.06). Additionally, the broilers fed diets contained butyrate salts showed the higher crypts depth of jejunal tissue than those from control and AGP groups, but villus height was not significantly affected. Moreover, supplementation with butyrate salts significantly decreased the population of lactic acid bacteria and increased the Salmonella sp. population in caecal digesta of broilers aged 20 days, whereas the non-significant effect was found in 35 days old birds.
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How to Cite
Passwang, U. ., Akaramathurakul, P.- on ., Songserm, T. ., & Paraksa, N. . (2020). Effects of butyrate salts on growth performances, intestinal morphological structure and caecal microbial population of broilers. Khon Kaen Agriculture Journal, 48(4), 733–742. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/251883
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บทความวิจัย (research article)
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References
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Abd El-Ghany, W. A., M. H. Awaad, S. A. Nasef, and A. F. Gaber. 2016. Effect of sodium butyrate on Salmonella enteritidis infection in broiler chickens. Asian J. Poult. Sci. 10: 104-110.
Ahsan U., O. Cengiz, E. Kuter, and M. E. A. Chacher. 2016. Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity. Worlds Poult. Sci. J. 72: 266-275.
Butaye, P., L. A. Devriese, and F. Haesebrouck. 2003. Antimicrobial growth promoters used in animal feed: Effects of less well known antibiotics on gram negative bacteria. Clin. Microbiol. Rev. 16: 175-188.
Czerwinski, J., O. Højberg, S. Smulikowska, R. M. Engberg, and A. Mieczkowska. 2012. Effects of sodium butyrate and salinomycin upon intestinal microbiota, mucosal morphology and performance of broiler chickens. Arch. Anim. Nutr. 66 : 102-116.
Guilloteau, P., L. Martin, V. Eckhart, R. Ducatelle, R. Zabielskiand, and F. V. Immerseel. 2010. From the gut to the peripheral tissues: the multiple effects of butyrate. Nutr. Res. Rev. 23: 366-384.
Halliwell, B and J.M.C. Gutteridge. 1989. Free Radical in Biology and Medicine. 2nd Edition. Oxford Science Publication, Clarendon.
Hamed, D.M. and A.M.A. Hassan. 2013. Acids supplementation to drinking water and their effects on Japanese quails experimentally challenged with Salmonella enteritidis. Res. Zool. 3: 15-22.
Hu, Z., and Y. Guo. 2007. Effects of dietary sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in chickens. Anim. Feed Sci. Technol. 132: 240-249.
Hutkins, R.W. and N.L. Nannen. 1993. pH homeostasis in lactic acid bacteria. J. Dairy Sci. 76: 2354-2365.
Imran, M., S. Ahmed, Y. A. Ditta, S. Mehmood, M. I. Khan, S. S. Gillani, Z. Rasool, M. L. Sohail, A. Mushtaq, and S. Umar. 2018. Effect of microencapsulated butyric acid supplementation on growth performance, ileal digestibility of protein, duodenal morphology and immunity in broilers. J. Hellenic. Vet. Med. Soc. 69: 1109-1116.
ISO 6579. 2002. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection of Salmonella spp. International Organization for Standardization, London.
ISO 7251. 2005. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection and Enumeration of Presumptive Escherichia coli- Most Probable Number Technique. International Organization for Standardization, London.
ISO 15214. 1998. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Enumeration of Mesophillic Lactic Acid Bacteria-Colony count Technique at 30 Degrees C. International Organization for Standardization, London.
Jozefiak, D., S. Kaczmarek, and A. Rutkowski. 2010. The effects of benzoic acid supplementation on the performance of broiler chickens. J. Anim. Physiol. Anim. Nutr. 94: 29-34.
Kaczmarek, S. A., A. Barri, M. Hejdysz, and A. Rutkowski. 2016. Effect of different doses of coated butyric acid on growth performance and energy utilization in broilers. Poult. Sci. 95: 851–859.
Kashket, E.R. 1987. Bioenergistics of lactic acid bacteria: cytoplasmic pH and osmotolerance. FEMS Microbiol. Rev. 46: 233-244.
Khadam, A., L. Soler, N. Everaert, and T. A. Niewold. 2014. Growth promotion in broilers by both oxytetracycline and Macleayacordata extract is based on their anti-inflammatory properties. Br. J. Nutr. 112: 1110–1118.
Kogut, M. H., X. Yin, J. Yuanand, and L. Bloom. 2017. Gut health in poultry. CAB Rev. 12: No.031.
Kotunia, A., J. Wolinski, D. Laubitz, M. Murkowski, V. Rome, P. Guilloteau, and R. Zabielski. 2004. Effect of sodium butyrate on the small intestine development in neonatal piglets fed by artificial sow. J. Physiol. Pharmacol. 55: 59–68.
Mahdavi, R., and M. Torki. 2009. Study on usage period of dietary protected butyric acid on performance, carcass characteristics, serum metabolite levels and humoral immune response of broiler chickens. J. Anim. Vet. Adv. 8: 1702-1709.
Miles, R. D., G. D. Butcher, P. R. Henry, and R. C. Littell. 2006. Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poult. Sci. 85: 476-485.
National Research Council (NRC). 1994. Nutrient Requirements of Poultry. 9th Edition. National Academy Press, Washington, DC.
Niewold, T. A. 2007. The non-antibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poult. Sci. 86: 605–609.
Nutrishatives. 2018. Top 5 best butyrate supplements for leaky gut cure. Gut health. Available: https://www.nutrishatives.com/best-butyrate-supplements-for-leaky-gut-cure/#What-are-the-Forms-of-Butyrate-in-Supplements.AccessedMar. 20, 2019.
Panda, A.K., S.V. Rama Rao, M.V.L.N. Raju, and G.S. Shyam. 2009. Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Aust. J. Anim. Sci. 22: 1026-1031.
Potten, C. S. 1998. Stem cell in gastrointestinal epithelium characteristics and death. Phil. Trans. R. Soc. Lond. 353: 821-830.
Roediger, W. E. 1982. Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterol. 83: 424–429.
Scheppach, W. 1994. Effects of short chain fatty acids on gut morphology and function. Gut. 35: 35-38.
Van Immerseel, F., J.B. Russell, M.D. Flythe, I. Gantois, L. Timbermont, F. Pasmans, F. Haesebrouck, and R. Ducatelle. 2006. The use of organic acids to combat Salmonella in poultry: a mechanistic explanation of the efficacy. Avian Pathol. 35: 182-188.
Weiss, W.P. and D.C. Mahan. 2008. Oxidative stress during the lifecycle of animals. J. Anim. Sci. 86:E-Suppl.2/J.Dairy Sci. 91: E-Suppl.1.
Wu, W., Z. Xiao, W. An, Y. Dong, and B. Zhang. 2018. Dietary sodium butyrate improves intestinal development and function by modulating the microbial community in broilers. PLosONE 13: 1-21.
Yang, Y., P. A. Iji, A. Kocher, L. L. Mikkelsen, and M. Choct. 2007. Effects of mannanoligosaccharide on growth performance, the development of gut microflora and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res.16: 280-288.
Zhang, W. H., Y. Jiang, Q. F. Zhu, F. Gao, S. F. Dai, J. Chen, and G. H. Zhou. 2011. Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br. Poult. Sci. 52: 292-301.
Abd El-Ghany, W. A., M. H. Awaad, S. A. Nasef, and A. F. Gaber. 2016. Effect of sodium butyrate on Salmonella enteritidis infection in broiler chickens. Asian J. Poult. Sci. 10: 104-110.
Ahsan U., O. Cengiz, E. Kuter, and M. E. A. Chacher. 2016. Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity. Worlds Poult. Sci. J. 72: 266-275.
Butaye, P., L. A. Devriese, and F. Haesebrouck. 2003. Antimicrobial growth promoters used in animal feed: Effects of less well known antibiotics on gram negative bacteria. Clin. Microbiol. Rev. 16: 175-188.
Czerwinski, J., O. Højberg, S. Smulikowska, R. M. Engberg, and A. Mieczkowska. 2012. Effects of sodium butyrate and salinomycin upon intestinal microbiota, mucosal morphology and performance of broiler chickens. Arch. Anim. Nutr. 66 : 102-116.
Guilloteau, P., L. Martin, V. Eckhart, R. Ducatelle, R. Zabielskiand, and F. V. Immerseel. 2010. From the gut to the peripheral tissues: the multiple effects of butyrate. Nutr. Res. Rev. 23: 366-384.
Halliwell, B and J.M.C. Gutteridge. 1989. Free Radical in Biology and Medicine. 2nd Edition. Oxford Science Publication, Clarendon.
Hamed, D.M. and A.M.A. Hassan. 2013. Acids supplementation to drinking water and their effects on Japanese quails experimentally challenged with Salmonella enteritidis. Res. Zool. 3: 15-22.
Hu, Z., and Y. Guo. 2007. Effects of dietary sodium butyrate supplementation on the intestinal morphological structure, absorptive function and gut flora in chickens. Anim. Feed Sci. Technol. 132: 240-249.
Hutkins, R.W. and N.L. Nannen. 1993. pH homeostasis in lactic acid bacteria. J. Dairy Sci. 76: 2354-2365.
Imran, M., S. Ahmed, Y. A. Ditta, S. Mehmood, M. I. Khan, S. S. Gillani, Z. Rasool, M. L. Sohail, A. Mushtaq, and S. Umar. 2018. Effect of microencapsulated butyric acid supplementation on growth performance, ileal digestibility of protein, duodenal morphology and immunity in broilers. J. Hellenic. Vet. Med. Soc. 69: 1109-1116.
ISO 6579. 2002. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection of Salmonella spp. International Organization for Standardization, London.
ISO 7251. 2005. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Detection and Enumeration of Presumptive Escherichia coli- Most Probable Number Technique. International Organization for Standardization, London.
ISO 15214. 1998. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Enumeration of Mesophillic Lactic Acid Bacteria-Colony count Technique at 30 Degrees C. International Organization for Standardization, London.
Jozefiak, D., S. Kaczmarek, and A. Rutkowski. 2010. The effects of benzoic acid supplementation on the performance of broiler chickens. J. Anim. Physiol. Anim. Nutr. 94: 29-34.
Kaczmarek, S. A., A. Barri, M. Hejdysz, and A. Rutkowski. 2016. Effect of different doses of coated butyric acid on growth performance and energy utilization in broilers. Poult. Sci. 95: 851–859.
Kashket, E.R. 1987. Bioenergistics of lactic acid bacteria: cytoplasmic pH and osmotolerance. FEMS Microbiol. Rev. 46: 233-244.
Khadam, A., L. Soler, N. Everaert, and T. A. Niewold. 2014. Growth promotion in broilers by both oxytetracycline and Macleayacordata extract is based on their anti-inflammatory properties. Br. J. Nutr. 112: 1110–1118.
Kogut, M. H., X. Yin, J. Yuanand, and L. Bloom. 2017. Gut health in poultry. CAB Rev. 12: No.031.
Kotunia, A., J. Wolinski, D. Laubitz, M. Murkowski, V. Rome, P. Guilloteau, and R. Zabielski. 2004. Effect of sodium butyrate on the small intestine development in neonatal piglets fed by artificial sow. J. Physiol. Pharmacol. 55: 59–68.
Mahdavi, R., and M. Torki. 2009. Study on usage period of dietary protected butyric acid on performance, carcass characteristics, serum metabolite levels and humoral immune response of broiler chickens. J. Anim. Vet. Adv. 8: 1702-1709.
Miles, R. D., G. D. Butcher, P. R. Henry, and R. C. Littell. 2006. Effect of antibiotic growth promoters on broiler performance, intestinal growth parameters, and quantitative morphology. Poult. Sci. 85: 476-485.
National Research Council (NRC). 1994. Nutrient Requirements of Poultry. 9th Edition. National Academy Press, Washington, DC.
Niewold, T. A. 2007. The non-antibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poult. Sci. 86: 605–609.
Nutrishatives. 2018. Top 5 best butyrate supplements for leaky gut cure. Gut health. Available: https://www.nutrishatives.com/best-butyrate-supplements-for-leaky-gut-cure/#What-are-the-Forms-of-Butyrate-in-Supplements.AccessedMar. 20, 2019.
Panda, A.K., S.V. Rama Rao, M.V.L.N. Raju, and G.S. Shyam. 2009. Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Aust. J. Anim. Sci. 22: 1026-1031.
Potten, C. S. 1998. Stem cell in gastrointestinal epithelium characteristics and death. Phil. Trans. R. Soc. Lond. 353: 821-830.
Roediger, W. E. 1982. Utilization of nutrients by isolated epithelial cells of the rat colon. Gastroenterol. 83: 424–429.
Scheppach, W. 1994. Effects of short chain fatty acids on gut morphology and function. Gut. 35: 35-38.
Van Immerseel, F., J.B. Russell, M.D. Flythe, I. Gantois, L. Timbermont, F. Pasmans, F. Haesebrouck, and R. Ducatelle. 2006. The use of organic acids to combat Salmonella in poultry: a mechanistic explanation of the efficacy. Avian Pathol. 35: 182-188.
Weiss, W.P. and D.C. Mahan. 2008. Oxidative stress during the lifecycle of animals. J. Anim. Sci. 86:E-Suppl.2/J.Dairy Sci. 91: E-Suppl.1.
Wu, W., Z. Xiao, W. An, Y. Dong, and B. Zhang. 2018. Dietary sodium butyrate improves intestinal development and function by modulating the microbial community in broilers. PLosONE 13: 1-21.
Yang, Y., P. A. Iji, A. Kocher, L. L. Mikkelsen, and M. Choct. 2007. Effects of mannanoligosaccharide on growth performance, the development of gut microflora and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res.16: 280-288.
Zhang, W. H., Y. Jiang, Q. F. Zhu, F. Gao, S. F. Dai, J. Chen, and G. H. Zhou. 2011. Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens. Br. Poult. Sci. 52: 292-301.
