Improving nutritional value of banana stem by using microorganism on chemical composition and digestibility of beef cattle using in vitro gas production technique

Main Article Content

Sineenart Polyorach
Ronachai Sitthigripong
Bpantamars Phadungchob
Rutsamee Nampukdee

Abstract

The objective of this study was to determine the improvement of nutritional values of banana tree using microorganisms on the digestibility of beef cattle by in vitro gas production technique. The experimental design was a 2X3 factorial arrangement in a completely randomized design (CRD) with 3 replications. Factor A (microorganism sources) composed of two levels which were yeast and effective microorganisms (EM). Factor B (silage time) consisted of three levels which were 0, 7 and 14 days. Cumulative gas production was recorded at 0, 2, 4, 6, 12, 24, 48, 72 and 96 h of incubation. It was found that increasing silage time of banana tree fermented with both microorganisms increased organic matter (OM), crude protein (CP) (P<0.05). However, dry matter (DM) and neutral detergent fiber (NDF) were decreased (P<0.05). Concerning sources of microorganisms, silage time and interaction between microorganism sources and silage time were not affected on the intercept value (a), gas production from the insoluble fraction (b), gas production rate constants for the insoluble fraction (c), potential extent of gas production (a+b) and cumulative gas production at 96 h. However, In vitro degradability tended to improve when silage time increased. In conclusion, microorganisms could improve the nutritional values of OM and CP. Moreover, they could increase digestibility when increased silage time. However, using microorganism fermentation for banana stem in in vivo trial should be further studied.

Article Details

How to Cite
Polyorach, S. ., Sitthigripong, R. ., Phadungchob, B. ., & Nampukdee, R. . (2021). Improving nutritional value of banana stem by using microorganism on chemical composition and digestibility of beef cattle using in vitro gas production technique. Khon Kaen Agriculture Journal, 50(1), 1–8. retrieved from https://li01.tci-thaijo.org/index.php/agkasetkaj/article/view/253300
Section
บทความวิจัย (research article)

References

ณรกมล เลาห์รอดพันธ์, ประวิทย์ ห่านใต้, ทศพร อินเจริญ, บุญฑริกา ปลั่งสูงเนิน, เสาวลักษณ์ แย้มหมื่นอาจ และ วิรัตน์ นาคเอี่ยม. 2562. การปรับปรุงคุณภาพของฟางข้าวด้วยหัวเชื้อพ.ด.1 และหัวเชื้ออีเอ็มต่อค่าองค์ประกอบทางเคมีและการย่อยสลายในหลอดทดลอง. แก่นเกษตร. 47: 777-782.

วาสนา ศิริแสน, และกิตติ วิรุณพันธุ์. 2557. กลยุทธ์การปรับปรุงคุณค่าทางโภชนะของเศษเหลือทางการเกษตรและโรงงานอุตสาหกรรมด้วยจุลินทรีย์โปรไบโอติกเพื่อเป็นอาหารสัตว์. การเกษตรราชภัฏ. 13(1): 1-10.

สินีนาฏ พลโยราช และ อรอนงค์ พวงชมภู. 2557. ผลของการเสริมแหล่งโปรตีนคุณภาพสูงต่อความสามารถในการย่อยได้ของโภชนะในกระเพาะรูเมนโดยวิธีถุงไนลอน. คณะทรัพยากรธรรมชาติ มหาวิทยาลัยเทคโนโลยีราชมงคลอีสาน วิทยาเขตสกลนคร, สกลนคร.

สินีนาฏ พลโยราช และ เมธา วรรณพัฒน์. 2558. ศักยภาพในการใช้ยีสต์เป็นแหล่งโปรไบโอติกส์ในสัตว์เคี้ยวเอื้อง. แก่นเกษตร. 43(1): 191-206.

อรอนงค์ ยามาเจริญ. 2558. ส่วนประกอบของต้นกล้วย. แหล่งข้อมูล:https://www.google.com/ Search ?ei=u93PXIeuEZzbz7sPt-CHwAY&q=%28.28. ค้นเมื่อ 3 พฤษภาคม 2563.

Association of Official Analytical Chemists (AOAC). 2016. Official Methods of Analysis. 20th Edition. AOAC, Gaithersburg, MD, USA.

Chen, X., W., Li, C. Gao, X. Zhang, B, Weng, and Y. Cai. 2017. Silage preparation and fermentation quality of kudzu, sugarcane top and their mixture treated with lactic acid bacteria, molasses and cellulase. Animal Science Journal. 88: 1715-1721.

Forsberg, C. W., E. Forano, and A. Chesson. 2000. Microbial adherence to the plant cell wall and enzymatic hydrolysis. P.79-97. In: P. B. Cronje. Ruminant physiology: Digestion, Metabolism, Growth and Reproduction. CABI Publishing, Wallingford, UK.

Huy, S., Tran, T. T. Hong., L. D. Ngoan., L. D. Phung, and K. Borin. 2018. Nutritive Value of Fermented Banana Pseudo Stem (Musa spp.) and Rice Bran by Saccharomyces cerevisiae. IJAIR. 7: 209-216.

Jouany, J.P. 2006. Optimizing rumen functions in the close-up transition period and early lactation to drive dry matter intake and energy balance in cows. Animal Reproduction Science. 96: 250-264.

Kimambo, A.E., and H.M.H. Muya. 1991. Rumen degradation of dry matter and organic matter of different part of the banana plant. Livestock Research for Rural Development. 3(3): 35-40.

Marie-Magdeleine, C., M. Boval, L. Philibert, A. Borde, and H. Archimède. 2010. Effect of banana foliage (Musa paradisiaca) on nutrition, parasite infection and growth of lambs. Livestock Science. 131: 234−239.

Menke, K.H. and H. Steingass. 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development. 28: 9-55.

Nampukdee, R., S. Polyorach, M. Wanapat, S. Kang, A. Cherdthong, P. Gunun, N. Gunun, and R. Sitthigripong. 2018. Effects of microbial fermented liquid (MFL) supplementation on gas production kinetics and digestibility using in vitro gas production technique. International Journal of Agricultural Technology. 14(7): 1495-1504

Oboh, G. 2006. Nutrient enrichment of cassava peels using a mixed culture Saccharomyces cerevisae and Lactobacillus spp. solid media fermentation techniques. Electronic Journal of Biotechnology. 9(1): 46-49.

Ørskov, E.R. and P. McDonald. 1979. The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. The Journal of Agricultural Science. 92: 499-503.

Polyorach, S., M. Wanapat, K. Phesatcha, and S. Kang. 2015. Effect of different levels of mangosteen peel powder supplement on the performance of dairy cows fed concentrate containing yeast fermented cassava chip protein. Tropical Animal Health and Production. 47: 1473–1480.

Roper, M.M., and J.K. Ladha. 1995. Biological N2 fixation by heterotrophic and phototrophic bacteria in association with straw. Plant and Soil. 174: 211-224.

SAS (1996) Statistical Analysis System. SAS User’s Guide: Statistics. SAS Institute Inc., Cary, USA.

Steel R. G. D., and J. H. Torrie. 1980. Principles and Procedures of Statistics. 2nd Edition. McGraw-Hill. New York.

Tilley J.M.A. and R.A. Terry 1963. A two-stage technique for the in vitro digestion of forage crops Current Contents. Journal of the British Grassland Society. 18: 104-111.

Van Soest, P. J., J. B. Robertson, and B.A. Lewism. 1991. Methods for dietary fiber neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74: 3583-3597.

Viswanathan, K., R. Kadirvel, and D. Chandrasekaran. 1989. Nutritive value of banana stalk (Musa cavendishi) as a feed for sheep. Animal Feed Science and Technology. 22: 93-113.

Wajizah, S., M. A. Akbar, and Y. Usman. 2019. In vitro digestibility assessment of banana stem silage (Musa paradisiaca) inoculated with EM-4 and different accelerators added as ruminant feed. Earth and Environmental Science. 387: 1-4.

Zhao, J., D. Zhihao, L. Junfeng, C. Lei, B. Yunfeng, J. Yushan, and S. Tao. 2019. Effects of lactic acid bacteria and molasses on fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro ruminal fermentation of rice straw silage. Animal Feed Science and Technology. 32(6): 783-791.