The efficacy of using exogenous enzymes treatment for improve soybean meal quality of nutritive value and nutrients digestibility in Pacific whiteleg shrimp (Litopenaeus vannamei)
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Abstract
Soybean meal (SBM) is widely used as a plant-based protein source in aquatic animal diets due to its high protein content, essential amino acids, and critical nutrients. However, its utilization is limited by anti-nutritional factors (ANFs), particularly phytic acid, which hinder nutrient absorption. This study investigated the use of a cocktail enzyme treatment to mitigate ANFs and improve SBM digestibility. The enzyme was applied at concentrations of 0, 10, 20, and 30 g/kg for treatment times of 0, 1, 2, 4, and 6 hours. A 4x5 factorial arrangement in a completely randomized design (CRD) with three replication runs was carried out for each treatment. The phytic acid content, in vitro protein and carbohydrate digestibility and chemical composition were analyzed. Results showed that enzymatic treatment significantly reduced phytic acid content within the first hour (P<0.05), with an optimal enzyme concentration of 20 g/kg. Chemical composition analysis revealed reduced fiber content (P<0.05). The highest of protein and carbohydrate digestibility, was observed at 4 and 2 hours, (P<0.05) respectively, compared to untreated SBM. These findings demonstrate that enzymatic pretreatment effectively reduces phytic acid and enhances nutrient digestibility in SBM. This method supports higher inclusion rates of SBM in aquafeed, reducing reliance on fishmeal and advancing sustainable aquaculture feed production. This study supports higher inclusion rates of SBM in aquafeed, reducing reliance on fishmeal to promote sustainability in the aquaculture industry.
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References
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