DNA markers related to anthocyanin contents and pericarp color in F2 progenies of the cross between Pathumthani 1 and Kham Noi
Main Article Content
Abstract
Rice with purple pericarp has high anthocyanin accumulation. Anthocyanin synthesis is controlled by OsB1 and OsDFR genes. In this study, relationship between the DNA markers of OsB1 and OsDFR genes and anthocyanin content was performed in 300 plants of F2 populations of the cross between white pericarp rice as female parent (Pathumthani 1) and purple pericarp rice as male parent (Kham Noi). Analysis of the relationship between genotype of DNA markers and phenotype of anthocyanin content and pericarp color by simple regression method was investigated. The result indicated that only OsB1, a functional marker, was highly related with anthocyanin content and pericarp color which had R2 equivalent to 31.9%. The segregation of pericarp color in F2 population showed color: colorless ratio of 3:1 consistent with the genotypic ratio of OsB1 gene, suggesting that pericarp pigmentation requires at least a single dominant allele of OsB1 gene. Anthocyanin content in F2 population was analyzed by HPLC method and the result revealed that the highest cyanidin-3-O-glucoside (C3G) content of 39.52 mg / 100 g of DW was found in F2 progeny with dark purple pericarp. Therefore, the DNA marker of OsB1 gene could be used to select rice with anthocyanin content and pericarp color. This marker will be beneficial for marker-assisted selection of rice plants at early stage of growth which will be convenient and shorten time for breeding rice with high nutritional value.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
กรมการข้าว. 2563. พันธุ์ข้าวปทุมธานี 1. แหล่งข้อมูล: http://www.ricethailand.go.th/Rkb/varieties/index.php-file=content.php&id=67.htm ค้นเมื่อ 15 ธันวาคม 2563.
จุฑาพร แสงประจักษ์. 2555. การใช้เครื่องหมายดีเอ็นเอสำหรับศึกษาความหลากหลายทางพันธุกรรม และการปรับปรุงพันธุ์ข้าว. แก่นเกษตร 40: 299-308.
รัชนี คงคาฉุยฉาย และริญ เจริญศิริ. 2553. คุณค่าโภชนาการของข้าวพันธุ์พื้นเมืองอำเภอกุดชุม จังหวัดยโสธร รายงานฉบับสมบูรณ์สถาบันโภชนาการ มหาวิทยาลัยมหิดล. นครปฐม.
อนงค์นาฏ หรี่จินดา, วราภรณ์ แสงทอง, แสงทอง พงษ์เจริญกิต และช่อทิพา สกูลสิงหาโรจน์. 2562. การใช้เครื่องหมายดีเอ็นเอของยีน OsB1 สำหรับใช้ตรวจสอบข้าวลูกผสมชั่วที่ 1 ของคู่ผสมระหว่างข้าวที่มีเยื่อหุ้มเมล็ดสีขาวและม่วง. น.181-189. ใน: การประชุมวิชาการพันธุศาสตร์แห่งชาติ ครั้งที่ 21. โรงแรมเดอะซายน์ พัทยา, ชลบุรี.
Ahmadiani, N., R. J. Robbins, T. M. Collins, and M. M. Giusti. 2016. Molar absorptivity (ε) and spectral characteristics of cyanidin-based anthocyanins from red cabbage. Food chemistry. 197: 900-906.
Guo, N., J. Wu, S. Zheng, F. Cheng, B. Liu, J. Liang, Y. Cui, and X. Wang. 2015. Anthocyanin profile characterization and quantitative trait locus mapping in zicaitai (Brassica rapa L. ssp. chinensis var. purpurea). Molecular Breeding. 35(5): 1-11.
Hwang, S.-K., and Y.-M. Kim. 2000. A simple and reliable method for preparation of cross-contamination-free plant genomic DNA for PCR-based detection of transgenes. Journal of Biochemistry and Molecular Biology. 33(6): 537-540.
Kim, J., H.-J. Lee, Y.-J. Jung, K.-K. Kang, W. Tyagi, M. Kovach, M. Sweeney, S. McCouch, and Y.-G. Cho. 2017. Functional properties of an alternative, tissue-specific promoter for rice NADPH-dependent dihydroflavonol reductase. PloS One. 12(8): e0183722.
Lee, J., R. W. Durst, and R. E. Wrolstad. 2005. Determination of total monomeric anthocyanin pigment
content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC international. 88(5): 1269-1278.
Lee, K. E., M. M. Rahman, J. B. Kim, and S. G. Kang. 2018. Genetic Analysis of Complementary Gene
Interactions of Pb and Pp Genes for the Purple Pericarp Trait in Rice. Journal of life science. 28(4): 398-407.
Lim, S.-H., and S.-H. Ha. 2013. Marker development for the identification of rice seed color. Plant Biotechnology Reports. 7(3): 391-398.
Nakai, K., Y. Inagaki, H. Nagata, C. MIYKZAKI, and S. IIDA. 1998. Molecular characterization of the gene for dihydroflavonol 4-reductase of japonica rice varieties. Plant Biotechnology. 15(4): 221-225.
Rahman, M. M., K. E. Lee, E. S. Lee, M. N. Matin, D. S. Lee, J. S. Yun, J. B. Kim, and S. G. Kang. 2013. The genetic constitutions of complementary genes Pp and Pb determine the purple color variation in pericarps with cyanidin-3-O-glucoside depositions in black rice. Journal of Plant Biology. 56(1): 24-31
Rahman, M. M., K. E. Lee, and S. G. Kang. 2016. Allelic gene interaction and anthocyanin biosynthesis of purple pericarp trait for yield improvement in black rice. Journal of Life Science. 26(6): 727-736.
Sakamoto, W., T. Ohmori, K. Kageyama, C. Miyazaki, A. Saito, M. Murata, K. Noda, and M. Maekawa. 2001. The Purple leaf (Pl) locus of rice: the Plw allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis. Plant and Cell Physiology. 42(9): 982-991.
Sakulsingharoj, C., P. Inta, R. Sukkasem, S. Pongjaroenkit, S. Chowpongpang, and V. Sangtong. 2016. Cloning and characterization of OSB1 gene controlling anthocyanin biosynthesis from Thai black rice. Genomics and Genetics. 9(1): 7-18.
Samyor, D., A. B. Das, and S. C. Deka. 2017. Pigmented rice a potential source of bioactive compounds: a review. International Journal of Food Science. 52(5): 1073-1081.
Somboon, P., C.Thebault Prom-u-thai, T. Pusadee, and S. Jamjod. 2017. Gene segregation for anthocyanin contents in F2 population between purple glutinous rice from highland and Pathum Thani 1 grown at lowland and highland locations. Journal of Agriculture. 33(3): 323-332.
Yang, X., X. Xia, Z. Zhang, B. Nong, Y. Zeng, Y. Wu, F. Xiong, Y. Zhang, H. Liang, and Y. Pan. 2019. Identification of anthocyanin biosynthesis genes in rice pericarp using PCAMP. Plant Biotechnology Journal. 17(9): 1700-1702.
Wang, C., and Q. Shu. 2007. Fine mapping and candidate gene analysis of purple pericarp gene Pb in rice (Oryza sativa L.). Chinese Science Bulletin. 52(22): 3097-3104.