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เถลิงเกียรติ สมนึก
ณัฏฐวรรณ สมนึก
สำเนาว์ เสาวกูล
กฤติมา เสาวกูล


This research aimed at determining the amount of vitamin E and sperm stimulate time to increase
semen quality of broad head catfish to hatchery. Randomize complete block design was designed for the
experiment, using male catfish with the average weight of 80 ± 1.03 g and the average length of 18 ± 1.90
cm Fish raised with three different levels of vitamin E (0, 600 and 1,000 mg kg-1) was divided into 3 groups
(200 fish each) with 3 replicates. Twenty of fish per group was randomly collected every 15 days for 90
days. Subsequently, first 10 fish was collected sperm sac and evaluated for sperm quality and other
ten fish was used to stimulate sperm before artificial breeding method with 5 different time courses
(0 1 2 3 and 4 min). The study indicated that sperm motility was statistically different (p <0.05). It was
found that vitamin E supplement at the level of 1,000 mg had the highest average motion (85.00 ± 6.24%).
Vitamin E supplement at the level of 600 mg kg-1 contributed the highest viability and sperm concentration
at 68.28±3.42% and 51.02±3.58 x 106 cells/ml, respectively. Fertilization rate and hatching rate obtained
from 600 mg kg-1 vitamin E and 2-min stimulation were 92.50 ± 3.35% and 79.50 ± 0.70%, respectively.

Therefore, supplementation of vitamin E in diet at 600 mg kg-1 with 2-min sperm stimulation with freshwater
before the artificial breeding of catfish, could enhance the hatching rate of broad head catfish.0.3


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