Enzymes production by plant pathogenic fungi causing foliar disease on ornamental plants
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Abstract
The research was carried out to investigate the ability of plant pathogenic fungi causing foliar
diseases in producing cell wall degrading enzymes of plant (cellulase, pectinase) and Fungi (chitinase)
on solid media. The tested pathogens (32 isolates) were isolated from diseased leaves of 13 Family of
ornamental plants in the area of King Mongkut’s Institute of Technology Ladkrabang, Bangkok. Activities
of cellulase and pectinase enzymes were determined by hydrolysis capacity (HC) value which was
the ratio of the hydrolysis zone and colony diameter. For chititnase, its activity was categorized based on
relative increase in diameter as well as intensity of purple color on colloidal chitin medium. Results revealed
that all tested fungal isolates (except Co 211-1) showed low potential to produce cellulase with HC value
of less than 1.00. Regard to pectinase production, presence of activity with low HC value (<1.00) was
detected in all tested fungal isolates except for all 19 isolates of Colletotrichum spp., Bipolaris sp.
(Bi 21-2) and Nigrospora sp. (Ni 12-4). For chitinolytic enzyme, all tested isolates of Colletotrichum spp.
(except Co 211-1) and all 2 tested Alternaria spp. presented moderate activity whereas all 4 tested
Curvularia spp. and Bipolaris sp. (Bi 21-2) showed low activity and the rest showed absent activity.
Furthermore, pathogenicity test was conducted in vitro using detached-leaf inoculation. Conclusively,
fungal isolates (from different host plants) within the same genera revealed the same potential to produce
cell wall degrading enzymes.
Article Details
King Mongkut's Agricultural Journal
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