Effect of Phosphorus Concentration on Growth and Antioxidant Properties in Bacopa monnieri (L.) Wettst. Growing in In Vitro Cultures
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Published:
Dec 25, 2020
Keywords:
Bacopa monnieri (L.) Wettst. phosphorus antioxidant in vitro growth
Main Article Content
Abstract
Bacopa monnieri (L.) Wettst. is known as a medical plant that can be used to improve human memory. Furthermore, its plant tissues contain a range of compounds that display antioxidant activities. In this study, the growth and antioxidant activity of explants of B. monnieri (L.) Wettst. cultivated in Murashige and Skoog (MS) medium with different phosphorus concentrations, ¼ P, ½ P, P and 2 P, were investigated. Explants on the ¼ P treatment demonstrated the maximum plant height, which was 39.39 mm. The highest number of leaves and nodes were found on the explants grown in the control treatment (P), and these were 16.05 leaves/explant and 7.50 nodes/explant. Furthermore, there were no significant differences in the number of roots and shoots among the treatments (P>0.05), which ranged over the values of 4.10-4.60 roots/explant and 1.63-1.88 shoots/explant respectively. As for the antioxidant activity, the double P treatment (2 P) produced the highest concentrations of TPC and TFC, expressed as 8.48 µg gallic/g and 16.44 mg luteolin/g, respectively. Compared to other treatments, the half P treatment (½ P) was observed to have produced explants with the highest saponin concentration (4,921 mg saponin/g) and the highest content of antioxidant activity as measured by ABTS assay, which was 79.66%. Finally, the said treatments did not produce any statistically significant differences in the DPPH assay results (P>0.05).
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How to Cite
Pinpirom, S. ., เลาหะวิสุทธิ์ น. ., & Ruangdej, U. . (2020). Effect of Phosphorus Concentration on Growth and Antioxidant Properties in Bacopa monnieri (L.) Wettst. Growing in In Vitro Cultures. King Mongkut’s Agricultural Journal, 38(4), 449–458. retrieved from https://li01.tci-thaijo.org/index.php/agritechjournal/article/view/248341
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Research Articles
King Mongkut's Agricultural Journal
References
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Sosa, M. D. L. M., Moroni, P., and Leary, N. O. 2018. A taxonomic revision of the genus Bacopa (Gratioleae, Plantaginaceae) in Argentina. Phytotaxa 336(1): 1-27.
Stewart, C. L., and Lovett-Doust, L. 2003. Effect of phosphorus treatment on growth and yield in the medicinal herb Calendula officinalis L. (Standard Pacific) under hydroponic cultivation. Canadian Journal of Plant Science 83(3): 611-617.
Vador, N., Vador, B., and Hole, R. 2012. Simple spectrophotometric methods for standardizing Ayurvedic formulation.
Indian Journal of Phamaceutical Sciences 74(2): 161-163.
Wu, J., and Zhong, J. J. 1999. Production of ginseng and its bioactive components in plant cell culture: Current technological and applied aspects. Journal of Biotechnology 67: 89-99.
ดิเรก ทองอร่าม. 2546. การปลูกพืชโดยไม่ใช้ดิน. ราชบุรี: ธรรมรักษ์การพิมพ์.
Akula, R., and Ravishankar, G. A. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior 6(11): 1720-1731.
Bhattacharya, S. K., Bhattacharya, A., Kumar, A., and Ghosal, S. 2000. Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytotherapy Research 14: 174-179.
Bramble, J. L., and Graves, D. J. 1990. Calcium and phosphate effects on growth and alkaloid production in Coffea arabica: experimental results and mathematical model. Biotechnology and Bioengineering 37: 859-868.
Chaudhari, K. S., Tiwari, N. R., Tiwari, R. R., and Sharma, R. S. 2017. Neurocognitive effect of nootropic drug Brahmi (Bacopa monnieri) in Alzheimer’s disease. Annals of Neurosciences 24: 111-122.
Chrysargyris, A., Panayiotou, C., and Tzortzakis, N. 2016. Nitrogen and phosphorus levels affected plant growth, essential oil composition and antioxidant status of lavender plant (Lavandula angustifolia Mill.). Industrial Crops and Products 83:
577-586.
Chrysargyris, A., Petropoulos, S. A., Fernandes, A., Barros, L., Tzortzakis, N., and Ferreira, I. C. F. R. 2019. Effect of phosphorus application rate on Mentha spicata L. grown in deep flow technique (DFT). Food Chemistry 276: 84-92.
Figas, A., Sowa, M. T., Sawilska, A., Bocian, K., and Figas, A. 2016. Effect of phosphorus on the growth and photosynthetic pigments content of Helichrysum arenarium (L.) Moench plantlets in in vitro cultures. Infrastructure and Ecology of Rural Areas 3(1): 697–704.
Ghosh, T., Maity, T. K., Sengupta, P., Dash, D. K., and Bose, A. 2008. Antidiabetic and In Vivo antioxidant activity of ethanolic extract of Bacopa monnieri Linn. aerial parts: A possible mechanism of action. Iranian Journal of Pharmaceutical Research 7(1): 61-68.
Hussain, A., Qarshi, I. A., Nazir, H., and Ullah, I. 2012. Plant tissue culture: Current status and opportunities. In Recent Advances in Plant in vitro Culture, A. Leva, and L. Rinaldi, eds. pp. 1-28. ISBN: 978-953-51-0787-3, DOI: 10.5772/52760.
Jain, P., Sharma, H., Basri, F., Priya, K., and Singh, P. 2017. Phytochemical analysis of Bacopa monnieri (L.) Wettst. and
their anti-fungal activities. Indian Journal of Traditional Knowledge 16(2): 310-318.
Lim, Y. Y., and Murtijaya, Y. 2007. Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods.
LWT - Food Science and Technology 40: 1664-1669.
Mathew, J., Paul, J., Nandhu, M. S., and Paulose, C. S. 2010. Bacopa monnieri and Bacoside-A for ameliorating epilepsy associated behavioral deficits. Fitoterapia 81: 315-322.
Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
Murthy, H. N., Lee, E. J., and Paek, K. Y. 2014. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tissue and Organ Culture 118: 1-16.
Nagella, P., and Murthy, H. N. 2011. Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tissue Organ Culture 104: 119-124.
Nell, M., Voetsch, M., Vierheilig, H., Steinkellner, S., ZitterlEglseer, K., Franz, C., and Novak, J. 2009. Effect of phosphorus uptake on growth and secondary metabolites of garden sage (Salvia officinalis L.). Journal of the Science of Food and Agriculture 89(6): 1090-1096.
Nilsson, J., Pillai, D., Onning, G., Persson, C., Nilsson, A., and Akesson, B. 2005. Comparison of the 2,29-azinobis-3-ethylbenzotiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) methods to assess the total antioxidant capacity in extracts of fruit and vegetables. Molecular Nutrition & Food Research 49: 239-246.
Ramezani, S., Rezaei, M. R., and Sotoudehnia, P. 2009. Improved growth, yield and essential oil content of basil grown under different levels of phosphorus sprays in the field. Journal of Applied Biological Sciences 3(2): 96-101.
Saad, A. I. M., and Elshahed, A. M. 2012. Plant tissue culture media. In Recent Advances in Plant in vitro Culture, A. Leva, and
L. Rinaldi, eds. pp. 29-40. ISBN: 978-953-51-0787-3, DOI: 10.5772/52760.
Shirazi, O. U., Khattak, M. M. A. K., Shukri, N. A. M., and Anuar, M. N. N. 2014. Determination of total Phenolic, flavonoid content and free radical scavenging activities of common herbs and spices. Journal of Pharmacognosy and Phytochemistry 3(3):
104-108.
Sosa, M. D. L. M., Moroni, P., and Leary, N. O. 2018. A taxonomic revision of the genus Bacopa (Gratioleae, Plantaginaceae) in Argentina. Phytotaxa 336(1): 1-27.
Stewart, C. L., and Lovett-Doust, L. 2003. Effect of phosphorus treatment on growth and yield in the medicinal herb Calendula officinalis L. (Standard Pacific) under hydroponic cultivation. Canadian Journal of Plant Science 83(3): 611-617.
Vador, N., Vador, B., and Hole, R. 2012. Simple spectrophotometric methods for standardizing Ayurvedic formulation.
Indian Journal of Phamaceutical Sciences 74(2): 161-163.
Wu, J., and Zhong, J. J. 1999. Production of ginseng and its bioactive components in plant cell culture: Current technological and applied aspects. Journal of Biotechnology 67: 89-99.
